Detailed information of TA system TA057443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 1603971..1604544
• Replicon: chromosome
• Accession: NZ_CP012901
• Organism: Pseudomonas aeruginosa strain N15-01092

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W5IUR0
• Locus tag: AOY09_RS07395
• Protein ID: WP_009618209.1
• Coordinates: 1604257..1604544 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W5ISI7
• Locus tag: AOY09_RS07390
• Protein ID: WP_009618210.1
• Coordinates: 1603971..1604270 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY09_RS07360	1599073..1599291	+	219	WP_011829925.1	AlpA family transcriptional regulator	-
AOY09_RS07365	1599332..1600198	+	867	WP_018076212.1	ParA family protein	-
AOY09_RS07370	1600182..1600418	+	237	WP_013982111.1	type II toxin-antitoxin system HicA family toxin	-
AOY09_RS07375	1600411..1602069	+	1659	WP_018076213.1	ParB family protein	-
AOY09_RS07380	1602087..1602647	+	561	WP_018076214.1	DUF2857 domain-containing protein	-
AOY09_RS07385	1602650..1603843	+	1194	WP_017244810.1	hypothetical protein	-
AOY09_RS07390	1603971..1604270	+	300	WP_009618210.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AOY09_RS07395	1604257..1604544	+	288	WP_009618209.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOY09_RS07400	1604573..1604764	+	192	WP_011805449.1	hypothetical protein	-
AOY09_RS07405	1604888..1605667	+	780	WP_017244811.1	TIGR03761 family integrating conjugative element protein	-
AOY09_RS07410	1605664..1606212	+	549	WP_017244812.1	DUF3158 family protein	-
AOY09_RS07415	1606209..1606658	+	450	WP_119163122.1	single-stranded DNA-binding protein	-
AOY09_RS07420	1606941..1608956	+	2016	WP_003141052.1	DNA topoisomerase III	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1584920..1663382	78462

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-58)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10854.46 Da
• Isoelectric Point: 8.4628

>T57443 WP_009618209.1 NZ_CP012901:1604257-1604544 [Pseudomonas aeruginosa]
VRVLEWREAARADLLAIVDYISDDNPDAAQRLKDDIEAKAAKLPERPKLYRPGRVAGTREMVVRANYVVVYMEDTRAVSI
LRVLHAAQQWPPARE

Nucleotide

• Length: 288 bp

>T57443 NZ_CP012901:1604257-1604544 [Pseudomonas aeruginosa]
GTGCGCGTACTTGAGTGGCGGGAAGCTGCCCGCGCCGACCTGCTGGCGATTGTTGACTACATCTCGGACGACAACCCCGA
TGCCGCGCAGCGGTTGAAGGACGACATCGAGGCCAAGGCCGCGAAGCTGCCGGAGCGCCCGAAGCTCTACCGGCCTGGCC
GCGTGGCCGGCACGCGGGAGATGGTCGTGCGTGCGAACTACGTGGTGGTCTACATGGAGGACACCCGCGCGGTGTCCATC
CTGCGCGTGTTGCACGCCGCGCAGCAGTGGCCGCCGGCACGGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10816.46 Da
• Isoelectric Point: 8.6579

>AT57443 WP_009618210.1 NZ_CP012901:1603971-1604270 [Pseudomonas aeruginosa]
MAAQTSMLHIRVDDKLKADAAEKLAGVGLTVSDAVRILLTRVVKEGALPAGLTADPEAYDAWFRAKVQEALADTRPPVPH
QQVMDEAQALIDRKRRART

Nucleotide

• Length: 300 bp

>AT57443 NZ_CP012901:1603971-1604270 [Pseudomonas aeruginosa]
ATGGCCGCACAGACTTCGATGCTGCACATCCGCGTGGACGATAAGCTCAAGGCCGATGCAGCCGAGAAACTCGCCGGGGT
CGGCCTCACCGTTTCGGACGCCGTGCGCATCCTGCTGACCCGTGTGGTCAAGGAAGGCGCACTGCCGGCTGGCCTCACGG
CCGACCCGGAGGCCTACGATGCCTGGTTCCGGGCGAAGGTCCAGGAGGCGCTGGCCGACACACGGCCGCCGGTGCCGCAC
CAGCAGGTCATGGATGAGGCGCAGGCCCTGATCGACAGGAAGCGGCGTGCGCGTACTTGA

Structures

Toxin

• AlphaFold DB: A0A5R9QF16

Antitoxin

• AlphaFold DB: A0A5R9QE11