Detailed information of TA system TA057432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-ChpS
• Location: 167614..168203
• Replicon: plasmid unnamed1
• Accession: NZ_CP012886
• Organism: Mycobacterium intracellulare subsp. chimaera strain AH16

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: AN480_RS28465
• Protein ID: WP_072501641.1
• Coordinates: 167614..167946 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: AN480_RS28470
• Protein ID: WP_069954322.1
• Coordinates: 167946..168203 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN480_RS28435	163620..164282	-	663	Protein_192	transposase	-
AN480_RS28440	164544..164846	-	303	WP_145929579.1	hypothetical protein	-
AN480_RS28445	164973..165095	-	123	WP_158514601.1	hypothetical protein	-
AN480_RS28455	165785..166294	-	510	WP_069954098.1	(3R)-hydroxyacyl-ACP dehydratase subunit HadC	-
AN480_RS28460	166297..166725	-	429	WP_072501577.1	(3R)-hydroxyacyl-ACP dehydratase subunit HadB	-
AN480_RS28465	167614..167946	-	333	WP_072501641.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AN480_RS28470	167946..168203	-	258	WP_069954322.1	antitoxin	Antitoxin
AN480_RS28480	168628..168960	+	333	WP_069954099.1	type II toxin-antitoxin system PemK/MazF family toxin	-
AN480_RS28485	169114..169845	-	732	WP_145929580.1	hypothetical protein	-
AN480_RS28490	170082..170582	-	501	Protein_201	transposase	-
AN480_RS30400	170921..171094	+	174	WP_083533558.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..437571	437571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (3-85)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11670.41 Da
• Isoelectric Point: 10.3742

>T57432 WP_072501641.1 NZ_CP012886:c167946-167614 [Mycobacterium intracellulare subsp. chimaera]
IERGDIYIVTLDPTSGHEQRGTRPVVIISPASFNRLTTTPVVLPITRGGNFARTAGFAVSLDGAGLRTTGVIRCDQPRAI
DIASRSGHRVERVPPAILDDVLAKVAAILS

Nucleotide

• Length: 333 bp

>T57432 NZ_CP012886:c167946-167614 [Mycobacterium intracellulare subsp. chimaera]
ATCGAGCGCGGCGACATCTATATCGTCACTCTGGATCCGACCTCGGGTCACGAGCAGCGCGGCACGCGGCCGGTGGTGAT
CATCTCGCCGGCGTCGTTTAATCGTTTGACCACAACACCGGTAGTGCTACCGATCACGCGAGGCGGGAACTTTGCCCGCA
CAGCAGGTTTCGCCGTTTCGTTGGATGGTGCGGGGCTGCGGACAACCGGCGTGATCCGCTGTGATCAACCCCGGGCGATC
GATATCGCTTCGCGCAGTGGGCACAGAGTTGAGCGGGTCCCCCCGGCGATTCTTGATGACGTCCTGGCCAAAGTCGCTGC
GATCTTGAGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9210.59 Da
• Isoelectric Point: 4.4100

>AT57432 WP_069954322.1 NZ_CP012886:c168203-167946 [Mycobacterium intracellulare subsp. chimaera]
VFTSKLRKVGGSVMLAIPPAVLDMLQLNPGQRVGLSVDGSHLVVDPQVRPRYTLDELLAQCDTSADITAEDQVWLDGQPI
GREVL

Nucleotide

• Length: 258 bp

>AT57432 NZ_CP012886:c168203-167946 [Mycobacterium intracellulare subsp. chimaera]
GTGTTCACGAGCAAGCTTCGGAAGGTCGGCGGATCGGTCATGCTGGCCATACCCCCGGCCGTGCTCGACATGCTCCAGCT
AAACCCTGGACAACGGGTCGGTCTATCGGTTGATGGGAGCCACCTCGTAGTCGATCCCCAAGTACGACCGCGCTACACGC
TCGATGAACTATTGGCACAGTGCGACACCTCAGCCGATATCACGGCCGAGGATCAGGTTTGGCTCGACGGCCAGCCTATC
GGCCGCGAGGTGCTCTAA