Detailed information of TA system TA057427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1406699..1407261
• Replicon: chromosome
• Accession: NZ_CP012885
• Organism: Mycobacterium intracellulare subsp. chimaera strain AH16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A081I0A5
• Locus tag: AN480_RS07030
• Protein ID: WP_036454801.1
• Coordinates: 1406893..1407261 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A081I0A4
• Locus tag: AN480_RS07025
• Protein ID: WP_036455532.1
• Coordinates: 1406699..1406896 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN480_RS06990	1401803..1402948	-	1146	WP_179945068.1	hypothetical protein	-
AN480_RS06995	1403078..1404151	+	1074	WP_042910932.1	redox-regulated ATPase YchF	-
AN480_RS07000	1404168..1404908	+	741	WP_042910933.1	aminoglycoside 3'-phosphotransferase	-
AN480_RS07005	1404996..1405280	+	285	WP_020821851.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AN480_RS07010	1405295..1405540	+	246	WP_042910934.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AN480_RS07015	1405576..1406127	+	552	WP_036454798.1	VOC family protein	-
AN480_RS07020	1406206..1406604	+	399	WP_036454800.1	VOC family protein	-
AN480_RS07025	1406699..1406896	+	198	WP_036455532.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AN480_RS07030	1406893..1407261	+	369	WP_036454801.1	type II toxin-antitoxin system VapC family toxin	Toxin
AN480_RS07035	1407444..1407785	+	342	WP_172417493.1	hypothetical protein	-
AN480_RS31560	1407871..1408230	+	360	WP_042910935.1	hypothetical protein	-
AN480_RS31460	1408415..1408946	+	532	Protein_1381	C40 family peptidase	-
AN480_RS07050	1409353..1409676	+	324	WP_036454806.1	antibiotic biosynthesis monooxygenase	-
AN480_RS07055	1409714..1410547	-	834	WP_036454808.1	SAM-dependent methyltransferase	-
AN480_RS07060	1410582..1411265	-	684	WP_036455537.1	hypothetical protein	-
AN480_RS07065	1411412..1411621	+	210	WP_072501223.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13435.74 Da
• Isoelectric Point: 6.4862

>T57427 WP_036454801.1 NZ_CP012885:1406893-1407261 [Mycobacterium intracellulare subsp. chimaera]
MILVDTAVWIDHLHTAELRLVKLLEIDQVGCHALVIEELALGSISRREVVLDLLANLRQFPTVQHAEILHLTGQRKLYGR
GLSAVDVHLMAAVALVEGAQLWTRDKRLKAASVDAGIALFDA

Nucleotide

• Length: 369 bp

>T57427 NZ_CP012885:1406893-1407261 [Mycobacterium intracellulare subsp. chimaera]
ATGATCCTCGTCGACACCGCGGTGTGGATCGATCATCTACACACCGCGGAGTTGCGGCTAGTCAAGCTGCTGGAGATCGA
CCAAGTTGGCTGTCATGCGCTGGTCATCGAAGAGCTGGCGCTGGGGTCGATCTCGCGACGTGAGGTGGTGCTCGACCTCC
TCGCGAATCTTCGCCAGTTTCCGACCGTCCAGCACGCCGAGATCCTTCACCTCACCGGTCAACGCAAACTGTATGGACGC
GGTCTCAGTGCGGTCGACGTCCATCTCATGGCCGCCGTCGCCCTGGTTGAAGGCGCGCAGCTGTGGACGAGAGACAAGCG
GTTGAAGGCTGCGTCGGTCGATGCGGGCATCGCACTCTTCGATGCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6871.88 Da
• Isoelectric Point: 11.6072

>AT57427 WP_036455532.1 NZ_CP012885:1406699-1406896 [Mycobacterium intracellulare subsp. chimaera]
MRTTVTIDDDLLAKAAELTGVRENVALLREGLQTLIRVESARRLAALGGTDAGAVAAPRRRRGTG

Nucleotide

• Length: 198 bp

>AT57427 NZ_CP012885:1406699-1406896 [Mycobacterium intracellulare subsp. chimaera]
ATGCGGACCACAGTCACGATCGACGACGACCTGTTGGCCAAGGCTGCTGAGTTGACCGGCGTGCGCGAGAACGTCGCGTT
GCTCCGCGAGGGATTGCAGACGCTGATCAGGGTGGAAAGCGCTCGCCGACTTGCCGCGCTCGGCGGGACGGATGCCGGGG
CGGTGGCCGCTCCCCGGCGCCGCCGTGGGACTGGATGA

Structures

Toxin

• AlphaFold DB: A0A081I0A5

Antitoxin

• AlphaFold DB: A0A081I0A4