Detailed information of TA system TA057424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 186797..187533
• Replicon: plasmid pKP1-19
• Accession: NZ_CP012884
• Organism: Klebsiella pneumoniae KP-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: KLP1_RS26725
• Protein ID: WP_003026803.1
• Coordinates: 186797..187279 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KLP1_RS26730
• Protein ID: WP_003026799.1
• Coordinates: 187267..187533 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KLP1_RS29225	181807..183061	+	1255	Protein_194	IS3 family transposase	-
KLP1_RS26710	183265..184263	+	999	WP_001610306.1	hypothetical protein	-
KLP1_RS26715	184267..185184	+	918	WP_158003452.1	hypothetical protein	-
KLP1_RS26720	185215..186618	-	1404	WP_004113090.1	ISNCY family transposase	-
KLP1_RS26725	186797..187279	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
KLP1_RS26730	187267..187533	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KLP1_RS26735	187709..187963	-	255	WP_004152108.1	hypothetical protein	-
KLP1_RS26740	188039..188296	-	258	WP_004152107.1	hypothetical protein	-
KLP1_RS26745	188345..188548	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
KLP1_RS26750	188582..188950	-	369	WP_004152105.1	hypothetical protein	-
KLP1_RS26755	188994..189488	-	495	WP_004152104.1	DNA-binding protein	-
KLP1_RS26760	189519..190093	-	575	Protein_205	hypothetical protein	-
KLP1_RS26765	190081..190350	-	270	WP_004152102.1	hypothetical protein	-
KLP1_RS28195	190665..191056	+	392	Protein_207	transcriptional regulator	-
KLP1_RS26775	191106..191466	+	361	Protein_208	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..194742	194742

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T57424 WP_003026803.1 NZ_CP012884:c187279-186797 [Klebsiella pneumoniae KP-1]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T57424 NZ_CP012884:c187279-186797 [Klebsiella pneumoniae KP-1]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT57424 WP_003026799.1 NZ_CP012884:c187533-187267 [Klebsiella pneumoniae KP-1]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT57424 NZ_CP012884:c187533-187267 [Klebsiella pneumoniae KP-1]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66