Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2906399..2906989 Replicon chromosome
Accession NZ_CP012871
Organism [Enterobacter] lignolyticus strain G5

Toxin (Protein)


Gene name pemK Uniprot ID A0A806X6Z1
Locus tag AO703_RS13515 Protein ID WP_062741467.1
Coordinates 2906399..2906731 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID E3G148
Locus tag AO703_RS13520 Protein ID WP_013365705.1
Coordinates 2906732..2906989 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO703_RS13495 2901820..2903307 + 1488 WP_062741464.1 aldehyde dehydrogenase PuuC -
AO703_RS13500 2903310..2904590 + 1281 WP_062741465.1 FAD-binding oxidoreductase -
AO703_RS13505 2904643..2906097 - 1455 WP_125451992.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
AO703_RS13515 2906399..2906731 - 333 WP_062741467.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AO703_RS13520 2906732..2906989 - 258 WP_013365705.1 antitoxin Antitoxin
AO703_RS13525 2907274..2907738 - 465 WP_062741468.1 hypothetical protein -
AO703_RS23355 2907621..2908079 - 459 WP_164504985.1 ash family protein -
AO703_RS13530 2908184..2908531 + 348 WP_013365700.1 HNH nuclease YajD -
AO703_RS13535 2908594..2909511 + 918 WP_062741469.1 M14 family metallocarboxypeptidase -
AO703_RS13540 2909579..2910499 - 921 WP_044611924.1 N-acetylmuramic acid 6-phosphate etherase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11771.63 Da        Isoelectric Point: 10.4487

>T57389 WP_062741467.1 NZ_CP012871:c2906731-2906399 [[Enterobacter] lignolyticus]
MERGEVWLVSLDPTAGHEQSGKRPVLIVSPAAFNQITRLPVVLPITTGGNFARTAGFAVSLDGVGTKTTGVIRCDQPRTI
DMAARHGKRLERLPDGIINDVLARLATILS

Download         Length: 333 bp

>T57389 NZ_CP012871:c2906731-2906399 [[Enterobacter] lignolyticus]
ATGGAGAGAGGAGAAGTCTGGCTCGTTTCGCTTGATCCTACCGCCGGACATGAGCAAAGCGGAAAACGCCCGGTGCTTAT
CGTTTCTCCGGCTGCGTTTAACCAAATCACGCGGTTACCCGTGGTTCTTCCGATAACAACGGGTGGGAACTTTGCCCGTA
CTGCGGGGTTCGCCGTGTCTCTGGATGGGGTGGGAACGAAAACAACGGGCGTCATTCGCTGCGATCAGCCGAGAACCATT
GATATGGCGGCCAGACACGGTAAGCGGCTTGAGCGTCTGCCTGACGGGATCATCAACGACGTGCTGGCAAGACTGGCGAC
CATCCTGAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9282.67 Da        Isoelectric Point: 4.4012

>AT57389 WP_013365705.1 NZ_CP012871:c2906989-2906732 [[Enterobacter] lignolyticus]
MYTTRLKKVGGSVMLAVPPAVLKTLALSADSEVGMTIDNGRIIIEPQKRPQYSLAELLAQCDPQAEISDEEREWVDSPAA
GRELL

Download         Length: 258 bp

>AT57389 NZ_CP012871:c2906989-2906732 [[Enterobacter] lignolyticus]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCGTCATGCTGGCCGTCCCGCCCGCCGTGCTGAAAACGCTGGCGCT
GTCGGCGGACAGCGAAGTCGGCATGACCATTGATAATGGTCGCATCATTATTGAACCGCAAAAGCGGCCTCAGTATTCGC
TGGCGGAGCTGCTGGCGCAGTGCGATCCGCAGGCTGAAATCAGCGATGAAGAGCGAGAATGGGTTGATTCGCCTGCCGCC
GGGAGGGAGCTTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A806X6Z1

Antitoxin

Source ID Structure
AlphaFold DB A0A806X5S9

References