Detailed information of TA system TA057377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 941..1557
• Replicon: chromosome
• Accession: NZ_CP012871
• Organism: [Enterobacter] lignolyticus strain G5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AO703_RS00015
• Protein ID: WP_013368310.1
• Coordinates: 1186..1557 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E3G5J9
• Locus tag: AO703_RS00010
• Protein ID: WP_013368311.1
• Coordinates: 941..1183 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO703_RS00010	941..1183	+	243	WP_013368311.1	ribbon-helix-helix protein, CopG family	Antitoxin
AO703_RS00015	1186..1557	+	372	WP_013368310.1	type II toxin-antitoxin system VapC family toxin	Toxin
AO703_RS00020	1598..2527	-	930	WP_062739895.1	formate dehydrogenase accessory protein FdhE	-
AO703_RS00025	2524..3159	-	636	WP_062739896.1	formate dehydrogenase cytochrome b556 subunit	-
AO703_RS00030	3156..4049	-	894	WP_013368307.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13547.69 Da
• Isoelectric Point: 6.7179

>T57377 WP_013368310.1 NZ_CP012871:1186-1557 [[Enterobacter] lignolyticus]
MEHMAVFDTNILIDLFNNRIEAADAIDSTATHRAISLITWMEVLVGARKYGQEAKTAAVMSAFEIIDVSREIAERSVLLR
VKHGMKLPDAIILATAQSKNCQLISRNTKDFAGISGVVSPYQL

Nucleotide

• Length: 372 bp

>T57377 NZ_CP012871:1186-1557 [[Enterobacter] lignolyticus]
ATGGAGCATATGGCAGTATTTGATACCAATATTCTCATCGACCTTTTCAACAATCGCATAGAGGCTGCGGATGCCATTGA
TAGCACCGCGACACATCGGGCAATAAGCCTCATCACCTGGATGGAAGTGTTGGTTGGCGCTCGTAAGTACGGTCAGGAAG
CCAAAACGGCGGCCGTAATGAGCGCGTTTGAGATTATCGATGTTTCGCGTGAGATTGCAGAACGCAGTGTATTACTTCGT
GTAAAACACGGGATGAAGCTTCCGGATGCCATCATTCTGGCTACCGCGCAAAGTAAAAACTGCCAACTTATCTCTCGTAA
TACGAAAGATTTTGCGGGTATCTCAGGGGTAGTTTCACCCTATCAGCTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9494.68 Da
• Isoelectric Point: 4.3324

>AT57377 WP_013368311.1 NZ_CP012871:941-1183 [[Enterobacter] lignolyticus]
MSHVVDLDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIEQYLDKQSSTVIREALGLWGNQEEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT57377 NZ_CP012871:941-1183 [[Enterobacter] lignolyticus]
ATGAGCCACGTGGTGGACCTTGATATGGGCAGGATTCTGATCGATTTGTCGGACGACGTCATTCAACGGCTGGATAACCT
TAAACAGTTGAGAAACCAGCCGCGCGCCGAGTTATTGCGTGAGGCCATTGAGCAATATCTCGATAAACAAAGCTCCACGG
TGATTCGGGAGGCTCTTGGTTTATGGGGAAATCAGGAAGAGGATGGGCTTGAATACGAACGTAAGCTTCGTGAGGAGTGG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A806X7M6