Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3131748..3132386 Replicon chromosome
Accession NZ_CP012870
Organism Escherichia coli strain K-12 substr. MG1655_TMP32XR2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag QR63_RS15390 Protein ID WP_000813794.1
Coordinates 3132210..3132386 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag QR63_RS15385 Protein ID WP_001270286.1
Coordinates 3131748..3132164 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QR63_RS15365 3126900..3127841 - 942 WP_001251304.1 ABC transporter permease -
QR63_RS15370 3127842..3128855 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
QR63_RS15375 3128873..3130018 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
QR63_RS15380 3130263..3131669 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
QR63_RS15385 3131748..3132164 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
QR63_RS15390 3132210..3132386 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
QR63_RS15395 3132608..3132838 + 231 WP_000494244.1 YncJ family protein -
QR63_RS15400 3132930..3134891 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
QR63_RS15405 3134964..3135500 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
QR63_RS15410 3135553..3136767 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T57372 WP_000813794.1 NZ_CP012870:c3132386-3132210 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T57372 NZ_CP012870:c3132386-3132210 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT57372 WP_001270286.1 NZ_CP012870:c3132164-3131748 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT57372 NZ_CP012870:c3132164-3131748 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References