Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2563711..2564542 Replicon chromosome
Accession NZ_CP012870
Organism Escherichia coli strain K-12 substr. MG1655_TMP32XR2

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag QR63_RS12470 Protein ID WP_000854814.1
Coordinates 2563711..2564085 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag QR63_RS12475 Protein ID WP_001285584.1
Coordinates 2564174..2564542 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QR63_RS12435 2559107..2560273 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
QR63_RS12440 2560392..2560865 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
QR63_RS12445 2561063..2562121 + 1059 WP_001200891.1 FUSC family protein -
QR63_RS12450 2562293..2562622 + 330 WP_000450409.1 DUF496 family protein -
QR63_RS12455 2562723..2562905 - 183 WP_001016348.1 hypothetical protein -
QR63_RS23955 2562977..2563105 + 129 Protein_2352 transposase domain-containing protein -
QR63_RS25955 2563394..2563507 - 114 WP_001161660.1 DUF957 domain-containing protein -
QR63_RS12465 2563520..2563714 - 195 WP_000988600.1 hypothetical protein -
QR63_RS12470 2563711..2564085 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
QR63_RS12475 2564174..2564542 - 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
QR63_RS12480 2564616..2564837 - 222 WP_000692323.1 DUF987 domain-containing protein -
QR63_RS25105 2564900..2565346 - 447 WP_000187523.1 RadC family protein -
QR63_RS12490 2565343..2566875 - 1533 WP_001350525.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T57361 WP_000854814.1 NZ_CP012870:c2564085-2563711 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T57361 NZ_CP012870:c2564085-2563711 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT57361 WP_001285584.1 NZ_CP012870:c2564542-2564174 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT57361 NZ_CP012870:c2564542-2564174 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References