Detailed information of TA system TA057348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 107600..108414
• Replicon: chromosome
• Accession: NZ_CP012870
• Organism: Escherichia coli strain K-12 substr. MG1655_TMP32XR2

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: QR63_RS00550
• Protein ID: WP_001054376.1
• Coordinates: 107600..107857 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: QR63_RS00555
• Protein ID: WP_001309181.1
• Coordinates: 107869..108414 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QR63_RS00525	102888..103994	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
QR63_RS00530	104059..105039	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
QR63_RS00535	105622..106862	-	1241	Protein_95	helicase YjhR	-
QR63_RS25425	106978..107223	+	246	Protein_96	GNAT family N-acetyltransferase	-
QR63_RS00550	107600..107857	+	258	WP_001054376.1	hypothetical protein	Toxin
QR63_RS00555	107869..108414	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
QR63_RS00560	108470..109216	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
QR63_RS24870	109385..109603	+	219	Protein_100	hypothetical protein	-
QR63_RS26005	109641..109757	+	117	Protein_101	VOC family protein	-
QR63_RS00565	110002..111123	+	1122	WP_000010829.1	M42 family metallopeptidase	-
QR63_RS00570	111120..111398	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
QR63_RS00575	111410..112723	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	97386..116638	19252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T57348 WP_001054376.1 NZ_CP012870:107600-107857 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T57348 NZ_CP012870:107600-107857 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT57348 WP_001309181.1 NZ_CP012870:107869-108414 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT57348 NZ_CP012870:107869-108414 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8