Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1600586..1601240 Replicon chromosome
Accession NZ_CP012868
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag QQ24_RS07890 Protein ID WP_000244777.1
Coordinates 1600833..1601240 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QQ24_RS07885 Protein ID WP_000354046.1
Coordinates 1600586..1600852 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQ24_RS07860 1595755..1596498 + 744 WP_000951964.1 SDR family oxidoreductase -
QQ24_RS07865 1596555..1597988 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
QQ24_RS07870 1598033..1598344 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
QQ24_RS07875 1598508..1599167 + 660 WP_000250274.1 hemolysin III family protein -
QQ24_RS07880 1599363..1600343 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QQ24_RS07885 1600586..1600852 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QQ24_RS07890 1600833..1601240 + 408 WP_000244777.1 toxin CptA Toxin
QQ24_RS07895 1601280..1601801 - 522 WP_001055874.1 flavodoxin FldB -
QQ24_RS07900 1601913..1602809 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QQ24_RS07905 1602834..1603544 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QQ24_RS07910 1603550..1605283 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T57294 WP_000244777.1 NZ_CP012868:1600833-1601240 [Escherichia coli str. K-12 substr. MG1655]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T57294 NZ_CP012868:1600833-1601240 [Escherichia coli str. K-12 substr. MG1655]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT57294 WP_000354046.1 NZ_CP012868:1600586-1600852 [Escherichia coli str. K-12 substr. MG1655]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT57294 NZ_CP012868:1600586-1600852 [Escherichia coli str. K-12 substr. MG1655]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References