Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 4819492..4820086 Replicon chromosome
Accession NZ_CP012831
Organism Pseudomonas fluorescens strain FW300-N2C3

Toxin (Protein)


Gene name relE Uniprot ID A0A0N9WLJ8
Locus tag AO356_RS21535 Protein ID WP_060741452.1
Coordinates 4819492..4819809 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2K4FP00
Locus tag AO356_RS21540 Protein ID WP_060741453.1
Coordinates 4819799..4820086 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO356_RS21495 4814530..4815741 + 1212 WP_060741448.1 O-succinylhomoserine sulfhydrylase -
AO356_RS21500 4815738..4816511 + 774 WP_060741449.1 SDR family oxidoreductase -
AO356_RS21520 4817181..4818464 - 1284 WP_060741450.1 FAD-binding oxidoreductase -
AO356_RS21530 4818808..4819452 + 645 WP_060741451.1 hypothetical protein -
AO356_RS21535 4819492..4819809 - 318 WP_060741452.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AO356_RS21540 4819799..4820086 - 288 WP_060741453.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AO356_RS21545 4820723..4821247 + 525 WP_060741454.1 hypothetical protein -
AO356_RS21550 4821373..4821987 + 615 WP_060741455.1 hypothetical protein -
AO356_RS21555 4822021..4822893 + 873 WP_203225759.1 hypothetical protein -
AO356_RS21560 4823202..4823402 + 201 WP_060741456.1 hypothetical protein -
AO356_RS32635 4823520..4824689 - 1170 WP_146042643.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4819492..4833440 13948


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(7-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12115.96 Da        Isoelectric Point: 9.8923

>T57264 WP_060741452.1 NZ_CP012831:c4819809-4819492 [Pseudomonas fluorescens]
MAVEYEVEWDPKALKELRKLDGTIRLQFLRKLQERQAGPRVPGDALHGMKDCYKIKLRGAGYRLVYRVEDERIVILVLAV
GKRERSSVYDSAKNRDSSGTDHDLK

Download         Length: 318 bp

>T57264 NZ_CP012831:c4819809-4819492 [Pseudomonas fluorescens]
ATGGCCGTTGAGTACGAAGTCGAGTGGGATCCGAAGGCGCTGAAAGAACTGCGAAAGCTCGACGGCACCATCCGCCTCCA
GTTCTTGCGAAAGCTCCAGGAACGGCAGGCCGGGCCACGAGTGCCAGGCGATGCACTTCACGGCATGAAGGATTGCTACA
AGATCAAATTGCGCGGCGCCGGCTACCGACTTGTGTACCGCGTTGAGGATGAACGGATTGTCATCTTGGTGCTGGCCGTC
GGCAAGCGGGAACGAAGCAGCGTCTACGATTCGGCCAAGAATCGGGATTCCTCAGGGACAGACCACGATTTGAAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10310.76 Da        Isoelectric Point: 4.4341

>AT57264 WP_060741453.1 NZ_CP012831:c4820086-4819799 [Pseudomonas fluorescens]
MQNILADVAVSVSELKKNPSSVLSNANGLPVAVLNHNRVMGYMVPANVYEAMVERLDELELVHLVKSRLDANETPVRVSL
DDLIGEAEADIANGR

Download         Length: 288 bp

>AT57264 NZ_CP012831:c4820086-4819799 [Pseudomonas fluorescens]
ATGCAAAATATTCTGGCTGACGTGGCTGTCAGCGTATCTGAACTGAAGAAGAATCCGTCTTCAGTCTTGAGCAACGCCAA
CGGGTTGCCCGTGGCCGTGCTGAACCACAACCGGGTCATGGGTTACATGGTCCCTGCCAACGTCTACGAGGCGATGGTTG
AGCGCTTGGATGAACTGGAACTGGTTCATTTGGTCAAGTCACGACTCGACGCCAACGAGACACCTGTTCGAGTATCTCTC
GACGATCTCATTGGCGAGGCCGAGGCAGATATCGCCAATGGCCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0N9WLJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A2K4FP00

References