Detailed information of TA system TA057263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4571957..4572729
• Replicon: chromosome
• Accession: NZ_CP012831
• Organism: Pseudomonas fluorescens strain FW300-N2C3

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: AO356_RS20510
• Protein ID: WP_081015391.1
• Coordinates: 4571957..4572445 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A0N9WBS3
• Locus tag: AO356_RS20515
• Protein ID: WP_060741283.1
• Coordinates: 4572436..4572729 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO356_RS20485	4569533..4569772	-	240	WP_060741278.1	hypothetical protein	-
AO356_RS20490	4569773..4570060	-	288	WP_060741279.1	ogr/Delta-like zinc finger family protein	-
AO356_RS31090	4569999..4570295	-	297	WP_081015474.1	DNA-binding protein	-
AO356_RS20500	4570398..4570745	+	348	WP_060741280.1	helix-turn-helix domain-containing protein	-
AO356_RS20505	4571069..4571875	+	807	WP_152032436.1	toll/interleukin-1 receptor domain-containing protein	-
AO356_RS20510	4571957..4572445	-	489	WP_081015391.1	hypothetical protein	Toxin
AO356_RS20515	4572436..4572729	-	294	WP_060741283.1	DUF1778 domain-containing protein	Antitoxin
AO356_RS32740	4572942..4573079	+	138	WP_160320219.1	hypothetical protein	-
AO356_RS20520	4573569..4575287	+	1719	WP_060741284.1	hypothetical protein	-
AO356_RS20525	4575343..4576197	+	855	WP_060741285.1	hypothetical protein	-
AO356_RS20530	4576501..4577067	+	567	WP_060741286.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4564034..4600522	36488

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (9-96)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17855.47 Da
• Isoelectric Point: 7.2420

>T57263 WP_081015391.1 NZ_CP012831:c4572445-4571957 [Pseudomonas fluorescens]
VELRAPEKLNDEHILDEFDSGVTSINDYLQRQARKAQAAKQAVVYVICRKDTPVVMGYYTLSSGSIARENVVPKSRQRNS
PSVHPVTLLGRMGITLEAQGQGFAIDLLQDAIERAISASNTIGSTAIIVHPLNERLSDFYTKYAGFIPCPELSPVTLMLP
LR

Nucleotide

• Length: 489 bp

>T57263 NZ_CP012831:c4572445-4571957 [Pseudomonas fluorescens]
GTGGAGCTAAGGGCTCCGGAAAAGCTGAACGATGAGCACATCTTGGATGAATTCGATTCTGGCGTGACATCGATCAACGA
TTACCTTCAAAGACAAGCGCGCAAAGCTCAAGCGGCAAAGCAGGCCGTTGTATACGTCATCTGCCGCAAGGATACGCCGG
TCGTCATGGGCTATTACACCCTGTCGAGCGGATCAATCGCACGCGAGAACGTCGTTCCGAAAAGCCGTCAACGTAATTCC
CCCAGCGTGCATCCCGTGACACTGCTGGGGCGCATGGGAATAACTCTGGAAGCACAAGGTCAAGGTTTCGCCATTGACTT
GCTGCAAGATGCGATTGAACGAGCGATTAGTGCGTCAAATACCATTGGCTCTACCGCTATAATTGTCCACCCGCTTAATG
AGCGTTTGTCGGACTTTTATACAAAATACGCAGGTTTTATACCGTGCCCGGAGCTTTCACCCGTCACATTAATGTTGCCG
CTCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11102.80 Da
• Isoelectric Point: 5.2166

>AT57263 WP_060741283.1 NZ_CP012831:c4572729-4572436 [Pseudomonas fluorescens]
MTVTIESVERSKPVPINMRADEKKRNLIDLAAAMSGRDRTSFILEAACQKAEEVILDKRLFLLDDVAFDAFEQAMEANPI
RGNECLHQLLERPKRWS

Nucleotide

• Length: 294 bp

>AT57263 NZ_CP012831:c4572729-4572436 [Pseudomonas fluorescens]
ATGACTGTAACAATTGAAAGTGTTGAAAGGTCAAAACCCGTTCCCATCAACATGAGAGCGGACGAAAAAAAACGAAACCT
GATCGATCTGGCAGCTGCTATGTCTGGTCGTGATCGAACCAGCTTTATCTTGGAAGCTGCTTGTCAGAAAGCAGAGGAAG
TCATCCTGGATAAGCGTCTGTTCCTTCTCGACGACGTTGCATTTGACGCATTTGAACAGGCAATGGAAGCCAACCCGATT
CGAGGTAACGAGTGCCTACATCAACTTCTCGAAAGGCCCAAACGGTGGAGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0N9WBS3