Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2235551..2236200 Replicon chromosome
Accession NZ_CP012831
Organism Pseudomonas fluorescens strain FW300-N2C3

Toxin (Protein)


Gene name vapC Uniprot ID A0A2K4FM26
Locus tag AO356_RS09900 Protein ID WP_060739625.1
Coordinates 2235781..2236200 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2K4FM86
Locus tag AO356_RS09895 Protein ID WP_060743091.1
Coordinates 2235551..2235781 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO356_RS09870 2231214..2231486 + 273 Protein_1960 DUF1016 family protein -
AO356_RS09875 2231586..2232191 + 606 WP_060739621.1 biliverdin-producing heme oxygenase -
AO356_RS09880 2232188..2234422 + 2235 WP_060739622.1 GAF domain-containing protein -
AO356_RS09885 2234463..2234909 + 447 WP_060739623.1 hypothetical protein -
AO356_RS09890 2234919..2235368 - 450 WP_060739624.1 hypothetical protein -
AO356_RS09895 2235551..2235781 + 231 WP_060743091.1 antitoxin Antitoxin
AO356_RS09900 2235781..2236200 + 420 WP_060739625.1 tRNA(fMet)-specific endonuclease VapC Toxin
AO356_RS09905 2236369..2236869 + 501 WP_060739626.1 hypothetical protein -
AO356_RS09910 2237170..2237943 + 774 WP_014336086.1 ABC transporter ATP-binding protein -
AO356_RS09915 2237957..2238712 + 756 WP_060739627.1 ABC transporter substrate-binding protein -
AO356_RS09920 2238791..2239486 + 696 WP_060739628.1 ABC transporter permease -
AO356_RS09925 2239483..2240172 + 690 WP_060739629.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(7-33)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15410.91 Da        Isoelectric Point: 7.9065

>T57261 WP_060739625.1 NZ_CP012831:2235781-2236200 [Pseudomonas fluorescens]
MIKFMLDTNICIFTIKNKPQAVREAFNLHHGQLSISAVTLMELIYGAEKSSAPEKNLAVIEGFCARLEVLPFDYEGAAHT
GMIRAELAKIGKPIGPYDQMIAGHARSRGLIVVTNNRREFERVPGLRIEDWTTATTPGL

Download         Length: 420 bp

>T57261 NZ_CP012831:2235781-2236200 [Pseudomonas fluorescens]
ATGATCAAGTTCATGCTAGATACCAACATTTGTATATTTACTATCAAGAACAAACCGCAGGCAGTCAGAGAAGCTTTCAA
CCTTCATCACGGCCAGCTTTCTATTAGCGCCGTGACGCTGATGGAGCTTATTTATGGCGCGGAAAAATCCTCTGCCCCTG
AGAAAAATCTTGCGGTCATAGAAGGGTTCTGTGCCCGGCTTGAAGTTTTACCTTTTGATTATGAAGGCGCAGCGCATACC
GGAATGATCCGAGCCGAGTTGGCAAAAATTGGCAAGCCGATCGGTCCCTACGACCAAATGATCGCAGGCCATGCCCGTTC
TCGCGGGCTGATCGTAGTGACTAACAATCGAAGGGAATTTGAAAGAGTCCCGGGCCTGCGCATCGAGGACTGGACCACAG
CGACGACGCCAGGGTTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8554.61 Da        Isoelectric Point: 4.3719

>AT57261 WP_060743091.1 NZ_CP012831:2235551-2235781 [Pseudomonas fluorescens]
MEQTTLFMSNRSQAVRLPKAVAMPNDVKRVDVIAIGRARVITPAGEAWDSWFDGDSVTADFMAEREQPADQEREPF

Download         Length: 231 bp

>AT57261 NZ_CP012831:2235551-2235781 [Pseudomonas fluorescens]
ATGGAACAAACCACCCTTTTCATGAGCAATCGCAGCCAGGCCGTCCGGCTACCCAAAGCAGTTGCGATGCCAAACGATGT
AAAGCGTGTAGACGTGATTGCCATAGGAAGAGCACGCGTCATCACACCAGCCGGCGAAGCGTGGGATAGCTGGTTCGATG
GAGACTCCGTAACAGCAGATTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGCCCTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K4FM26

Antitoxin

Source ID Structure
AlphaFold DB A0A2K4FM86

References