57260

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 1868866..1869462 Replicon chromosome
Accession NZ_CP012831
Organism Pseudomonas fluorescens strain FW300-N2C3

Toxin (Protein)


Gene name PumA Uniprot ID A0A2K4FVL4
Locus tag AO356_RS08320 Protein ID WP_060739368.1
Coordinates 1868866..1869168 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2K4FWQ4
Locus tag AO356_RS08325 Protein ID WP_024617344.1
Coordinates 1869172..1869462 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO356_RS08300 1864272..1865177 + 906 WP_060739364.1 zeta toxin family protein -
AO356_RS08305 1865647..1866858 + 1212 WP_060739365.1 bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase -
AO356_RS08310 1866851..1867948 + 1098 WP_060739366.1 cobalt-precorrin-5B (C(1))-methyltransferase -
AO356_RS08315 1867945..1868673 + 729 WP_060739367.1 cobalt-precorrin-6A reductase -
AO356_RS08320 1868866..1869168 + 303 WP_060739368.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AO356_RS08325 1869172..1869462 + 291 WP_024617344.1 putative addiction module antidote protein Antitoxin
AO356_RS08330 1869684..1870082 + 399 WP_060739369.1 DUF2946 domain-containing protein -
AO356_RS08335 1870129..1870608 + 480 WP_060739370.1 copper chaperone PCu(A)C -
AO356_RS08340 1870648..1871070 + 423 WP_081015333.1 DUF2946 domain-containing protein -
AO356_RS08345 1871153..1873279 + 2127 WP_060739372.1 TonB-dependent copper receptor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Uncharacterized protein HI1420

(4-95)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11196.73 Da        Isoelectric Point: 8.0229

>T57260 WP_060739368.1 NZ_CP012831:1868866-1869168 [Pseudomonas fluorescens]
MIEFEQSRVFSTWLDGLKDIAGKARIIARLRSAEHGNFGDCEPVGNAVYEMRIHSGPGYRVYFSRQGSVLYLLLVGGDKS
TQKRDIARAVQMAQTMGNEE

Download         Length: 303 bp

>T57260 NZ_CP012831:1868866-1869168 [Pseudomonas fluorescens]
ATGATCGAATTTGAACAGTCCCGAGTATTCTCAACCTGGCTTGACGGGTTGAAGGACATTGCTGGGAAAGCTCGCATCAT
TGCCAGGCTCCGATCGGCAGAGCATGGGAATTTTGGTGATTGCGAGCCGGTGGGTAACGCAGTGTATGAGATGCGTATTC
ACTCGGGTCCTGGCTACAGAGTGTACTTCTCCCGTCAAGGCTCCGTGCTTTATTTGCTGCTTGTGGGAGGTGACAAATCG
ACTCAGAAACGGGATATCGCACGCGCTGTTCAAATGGCGCAAACCATGGGTAATGAGGAGTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10403.92 Da        Isoelectric Point: 4.8096

>AT57260 WP_024617344.1 NZ_CP012831:1869172-1869462 [Pseudomonas fluorescens]
MTEEFTRFDAATYLKTPEEMAAYLDACFEEDAGDGVLVRAALNDIARAKGMTQIARDAGLGRESLYKALGSTGNPEFTTI
MKVMKALGLRLHATAI

Download         Length: 291 bp

>AT57260 NZ_CP012831:1869172-1869462 [Pseudomonas fluorescens]
ATGACTGAAGAATTCACACGTTTCGATGCAGCCACCTACCTCAAGACGCCTGAGGAAATGGCCGCTTATCTGGATGCCTG
TTTTGAAGAGGATGCGGGTGACGGCGTACTGGTTCGGGCAGCTCTTAACGATATTGCCAGGGCAAAGGGCATGACGCAGA
TTGCTCGTGATGCAGGCCTGGGACGAGAGAGTCTTTACAAAGCACTGGGAAGTACGGGCAATCCGGAGTTCACAACTATC
ATGAAAGTCATGAAAGCTTTGGGCTTGAGGTTACATGCGACGGCCATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K4FVL4

Antitoxin

Source ID Structure
AlphaFold DB A0A2K4FWQ4

References