Detailed information of TA system TA057257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4985742..4986256
• Replicon: chromosome
• Accession: NZ_CP012830
• Organism: Pseudomonas fluorescens strain FW300-N2E3

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: AO353_RS22760
• Protein ID: WP_203226141.1
• Coordinates: 4985742..4985963 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A2N8BVV6
• Locus tag: AO353_RS22765
• Protein ID: WP_054596996.1
• Coordinates: 4985966..4986256 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO353_RS22750	4981131..4983755	+	2625	WP_054596994.1	glucosidase	-
AO353_RS22755	4984578..4985552	+	975	WP_054596995.1	zeta toxin family protein	-
AO353_RS22760	4985742..4985963	+	222	WP_203226141.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AO353_RS22765	4985966..4986256	+	291	WP_054596996.1	putative addiction module antidote protein	Antitoxin
AO353_RS22770	4986520..4988367	-	1848	WP_162491292.1	amidohydrolase	-
AO353_RS22775	4988675..4988989	-	315	WP_019690779.1	SCP2 sterol-binding domain-containing protein	-
AO353_RS22780	4989040..4989750	-	711	WP_054596998.1	phosphoglycerate mutase family protein	-
AO353_RS22785	4989956..4990978	+	1023	WP_054596999.1	protease SohB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-72)

Antitoxin

• Uncharacterized protein HI1420 (7-92)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 7682.88 Da
• Isoelectric Point: 10.8536

>T57257 WP_203226141.1 NZ_CP012830:4985742-4985963 [Pseudomonas fluorescens]
IAIARRIDRATTGNLGDVKPVGEGVSEVRVDVGAGYRVYFTMRGGVVVVLLAGGGKSTQPSDIRQAKRLAKEV

Nucleotide

• Length: 222 bp

>T57257 NZ_CP012830:4985742-4985963 [Pseudomonas fluorescens]
ATTGCCATCGCTCGACGCATTGATCGAGCCACAACGGGCAACCTGGGCGATGTGAAGCCAGTAGGTGAAGGTGTATCCGA
GGTGCGCGTTGATGTCGGGGCTGGATACCGCGTGTATTTCACAATGCGCGGCGGTGTTGTCGTGGTCCTGCTGGCTGGCG
GGGGCAAGTCCACTCAACCTTCCGATATCCGGCAGGCCAAAAGATTGGCAAAGGAGGTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10328.81 Da
• Isoelectric Point: 4.5720

>AT57257 WP_054596996.1 NZ_CP012830:4985966-4986256 [Pseudomonas fluorescens]
MSDTLMPFDMAELLDTDEAVGEYLSQVLAEGDTDEFIRAVGYVAKARGMAQIANDSGLGRASLYKAFSPGAKPRFDTVIK
VMRAMGIELHATVGRA

Nucleotide

• Length: 291 bp

>AT57257 NZ_CP012830:4985966-4986256 [Pseudomonas fluorescens]
ATGTCCGATACCCTTATGCCGTTTGATATGGCGGAGCTCCTGGACACTGACGAAGCGGTCGGTGAATACCTCTCTCAGGT
TCTGGCCGAGGGCGACACTGACGAGTTCATCCGTGCAGTTGGATACGTCGCAAAAGCGCGCGGCATGGCCCAGATTGCGA
ATGACTCAGGCCTTGGCCGGGCGAGCTTGTACAAAGCATTTTCGCCAGGCGCCAAGCCACGCTTCGACACGGTGATTAAA
GTGATGCGTGCGATGGGCATTGAGCTCCACGCCACCGTTGGGCGTGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N8BVV6