Detailed information of TA system TA057256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3857872..3858493
• Replicon: chromosome
• Accession: NZ_CP012830
• Organism: Pseudomonas fluorescens strain FW300-N2E3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AO353_RS29865
• Protein ID: WP_081013750.1
• Coordinates: 3858311..3858493 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2N8C0W8
• Locus tag: AO353_RS17475
• Protein ID: WP_054596083.1
• Coordinates: 3857872..3858279 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO353_RS17445	3853403..3853927	+	525	WP_054596079.1	DUF4142 domain-containing protein	-
AO353_RS17450	3854138..3854677	-	540	WP_054596080.1	hypothetical protein	-
AO353_RS17455	3854800..3855303	-	504	WP_054596081.1	inclusion body family protein	-
AO353_RS17460	3855728..3856150	-	423	Protein_3460	DUF1501 domain-containing protein	-
AO353_RS17465	3856384..3856713	+	330	WP_019692252.1	YXWGXW repeat-containing protein	-
AO353_RS17470	3857066..3857836	+	771	WP_054596082.1	trans-aconitate 2-methyltransferase	-
AO353_RS17475	3857872..3858279	-	408	WP_054596083.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AO353_RS29865	3858311..3858493	-	183	WP_081013750.1	type II toxin-antitoxin system HicA family toxin	Toxin
AO353_RS17480	3858820..3859158	-	339	WP_054596084.1	hypothetical protein	-
AO353_RS17485	3859607..3861064	+	1458	WP_054596085.1	cytochrome c oxidase subunit II	-
AO353_RS17490	3861113..3862888	+	1776	WP_054596086.1	cytochrome c oxidase subunit I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6841.07 Da
• Isoelectric Point: 10.7851

>T57256 WP_081013750.1 NZ_CP012830:c3858493-3858311 [Pseudomonas fluorescens]
VNSRYLITQIVADGWYLVRVRGSHHHFKHPVKPGLVTVPHPKKDLLTKTAVSILQQALLY

Nucleotide

• Length: 183 bp

>T57256 NZ_CP012830:c3858493-3858311 [Pseudomonas fluorescens]
GTGAATAGCCGATATTTGATAACCCAAATCGTCGCAGACGGCTGGTATCTGGTACGGGTCAGGGGCAGCCATCACCACTT
CAAGCATCCTGTGAAGCCCGGTCTGGTCACCGTCCCACACCCGAAAAAGGATTTACTGACCAAAACGGCCGTCAGCATCC
TGCAACAGGCCCTGCTGTATTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14528.47 Da
• Isoelectric Point: 4.4564

>AT57256 WP_054596083.1 NZ_CP012830:c3858279-3857872 [Pseudomonas fluorescens]
MLYPIAISMGDNEHAWGVEVPDIPGCFSAGDDLDDAIAMAREAIEGHFEILAEDGSAIPPANKVTVHAANPQYAGATWAL
VDIDVTKYLGKAQKLNITLPGYLLTRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT57256 NZ_CP012830:c3858279-3857872 [Pseudomonas fluorescens]
ATGCTTTACCCAATTGCGATTTCAATGGGCGATAACGAACACGCCTGGGGCGTGGAAGTGCCGGATATTCCGGGCTGTTT
CTCGGCCGGTGATGACCTGGACGATGCGATCGCCATGGCGCGCGAAGCCATCGAAGGCCACTTCGAGATACTCGCCGAAG
ACGGCTCGGCGATTCCACCCGCCAACAAGGTCACCGTACACGCGGCCAATCCGCAGTACGCCGGCGCTACCTGGGCATTG
GTGGACATCGATGTCACCAAGTACCTGGGCAAGGCTCAGAAACTCAACATCACCCTGCCCGGCTATCTGCTGACCCGTAT
CGACGAATACGTGCTGCATCACCCGGAAGAAAAAAGCCGTTCCGGCTTCCTCGCCTCGGCGGCGTTGAAAGTGTTGCAAC
AGGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N8C0W8