Detailed information of TA system TA057255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 3850786..3851299
• Replicon: chromosome
• Accession: NZ_CP012830
• Organism: Pseudomonas fluorescens strain FW300-N2E3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AO353_RS29855
• Protein ID: WP_081013749.1
• Coordinates: 3850786..3851070 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N8BS75
• Locus tag: AO353_RS17435
• Protein ID: WP_054596077.1
• Coordinates: 3851060..3851299 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO353_RS17405	3845792..3846373	+	582	WP_054596072.1	DJ-1/PfpI family protein	-
AO353_RS17410	3846388..3846693	+	306	WP_054596073.1	ribbon-helix-helix domain-containing protein	-
AO353_RS17415	3846876..3847220	+	345	WP_054596074.1	YegP family protein	-
AO353_RS29845	3847276..3847440	+	165	Protein_3448	cupin domain-containing protein	-
AO353_RS17420	3847614..3848633	-	1020	WP_054598258.1	DUF4917 family protein	-
AO353_RS17425	3848789..3849937	+	1149	WP_054596075.1	L-threonine dehydrogenase	-
AO353_RS17430	3850032..3850772	+	741	WP_054596076.1	hypothetical protein	-
AO353_RS29855	3850786..3851070	-	285	WP_081013749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AO353_RS17435	3851060..3851299	-	240	WP_054596077.1	ribbon-helix-helix protein, CopG family	Antitoxin
AO353_RS31380	3851426..3851602	+	177	WP_173859970.1	hypothetical protein	-
AO353_RS29860	3851642..3851824	+	183	Protein_3455	LysE family translocator	-
AO353_RS17440	3851930..3853273	+	1344	WP_054596078.1	GGDEF domain-containing protein	-
AO353_RS17445	3853403..3853927	+	525	WP_054596079.1	DUF4142 domain-containing protein	-
AO353_RS17450	3854138..3854677	-	540	WP_054596080.1	hypothetical protein	-
AO353_RS17455	3854800..3855303	-	504	WP_054596081.1	inclusion body family protein	-
AO353_RS17460	3855728..3856150	-	423	Protein_3460	DUF1501 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10842.61 Da
• Isoelectric Point: 10.6100

>T57255 WP_081013749.1 NZ_CP012830:c3851070-3850786 [Pseudomonas fluorescens]
MRVEWLKTALKNIDDEAAYIALESPKAAADFVKAIFTSIEHLAQFPAIGREGRLPGTREWVLADRPYVIPYRVRQGRLQV
LRVFHTRRLPPGTW

Nucleotide

• Length: 285 bp

>T57255 NZ_CP012830:c3851070-3850786 [Pseudomonas fluorescens]
ATGCGCGTTGAGTGGCTGAAAACCGCACTAAAAAATATTGATGACGAAGCCGCTTACATCGCGCTGGAAAGTCCAAAAGC
CGCAGCTGACTTCGTCAAAGCCATCTTCACCAGTATCGAGCACCTTGCCCAATTTCCTGCGATTGGCCGTGAAGGCCGTC
TGCCCGGCACTCGAGAGTGGGTACTAGCCGATCGCCCCTACGTCATCCCTTATAGAGTCCGCCAAGGGCGTTTGCAGGTT
CTGCGAGTGTTCCACACCCGCCGTCTACCCCCCGGAACATGGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8774.84 Da
• Isoelectric Point: 4.5994

>AT57255 WP_054596077.1 NZ_CP012830:c3851299-3851060 [Pseudomonas fluorescens]
MSVMSLRLPDEIAETLANLAKATGRSKSFLAVDALREYLAREAWQIEEIQHALKEADAGDFASDEDVNALARKWANNAR

Nucleotide

• Length: 240 bp

>AT57255 NZ_CP012830:c3851299-3851060 [Pseudomonas fluorescens]
ATGTCCGTCATGTCTTTGCGCCTGCCAGACGAAATCGCCGAGACTCTCGCCAATTTGGCTAAAGCGACAGGCCGCAGCAA
ATCTTTTCTCGCCGTAGATGCGCTACGTGAATACCTGGCTCGAGAGGCCTGGCAAATCGAAGAAATCCAACATGCCTTAA
AAGAGGCTGACGCTGGAGATTTTGCCAGTGACGAAGACGTAAATGCCTTGGCCCGCAAGTGGGCAAACAATGCGCGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N8BS75