Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2644189..2644826 Replicon chromosome
Accession NZ_CP012830
Organism Pseudomonas fluorescens strain FW300-N2E3

Toxin (Protein)


Gene name vapC Uniprot ID A0A2N8BYT7
Locus tag AO353_RS12010 Protein ID WP_054595146.1
Coordinates 2644189..2644596 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1J0EKV4
Locus tag AO353_RS12015 Protein ID WP_054595147.1
Coordinates 2644596..2644826 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO353_RS11990 2639856..2640551 - 696 WP_054595142.1 ABC transporter permease -
AO353_RS11995 2640630..2641385 - 756 WP_054595143.1 ABC transporter substrate-binding protein -
AO353_RS12000 2641399..2642172 - 774 WP_054595144.1 ABC transporter ATP-binding protein -
AO353_RS12005 2642728..2644119 + 1392 WP_054595145.1 GABA permease -
AO353_RS12010 2644189..2644596 - 408 WP_054595146.1 tRNA(fMet)-specific endonuclease VapC Toxin
AO353_RS12015 2644596..2644826 - 231 WP_054595147.1 antitoxin Antitoxin
AO353_RS12020 2645017..2645469 - 453 WP_054595148.1 hypothetical protein -
AO353_RS12025 2645504..2646496 - 993 WP_054595149.1 alpha/beta fold hydrolase -
AO353_RS12030 2646643..2647047 - 405 WP_054595150.1 hypothetical protein -
AO353_RS12035 2647382..2647840 + 459 WP_054598216.1 hypothetical protein -
AO353_RS12045 2648129..2648401 - 273 WP_054595151.1 oxidative damage protection protein -
AO353_RS12050 2648398..2649465 - 1068 WP_054595152.1 A/G-specific adenine glycosylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-124)

Antitoxin

SpoVT-AbrB domain

(7-43)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15134.53 Da        Isoelectric Point: 6.7510

>T57254 WP_054595146.1 NZ_CP012830:c2644596-2644189 [Pseudomonas fluorescens]
MIKYMLDTNICIFTIKNKPQVVREAFNRHHGQLCISAVTLMELIYGAEKSAAPEKNLAVIEGFVARLEVLPFDYEAAAHT
GMIRSELAKAGTPIGPYDHMIAGHARSQGFIVVTNNLREFERVPGLRVEDWVHLD

Download         Length: 408 bp

>T57254 NZ_CP012830:c2644596-2644189 [Pseudomonas fluorescens]
ATGATCAAATACATGCTCGATACCAACATCTGCATTTTTACCATCAAGAACAAACCACAGGTCGTACGCGAAGCGTTCAA
TCGCCATCACGGCCAGCTGTGTATAAGCGCAGTCACGCTGATGGAATTGATTTACGGCGCTGAAAAATCGGCGGCGCCGG
AAAAGAACCTGGCCGTAATCGAAGGTTTTGTAGCTCGTCTTGAAGTCCTGCCCTTCGACTACGAAGCCGCTGCCCACACC
GGAATGATTCGCTCGGAACTTGCAAAGGCGGGCACACCGATAGGCCCTTACGACCACATGATCGCAGGACACGCGCGCTC
ACAAGGGTTCATTGTGGTGACGAATAATCTTCGGGAATTTGAGCGTGTTCCCGGATTGCGCGTAGAGGATTGGGTACACC
TCGATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8629.63 Da        Isoelectric Point: 4.2683

>AT57254 WP_054595147.1 NZ_CP012830:c2644826-2644596 [Pseudomonas fluorescens]
MEQTTLFMSNRSQAVRLPKAVAMPEDVKRVNVVAIGRARIITPADEAWDSWFDGDNVTADFMADREQPADQERESF

Download         Length: 231 bp

>AT57254 NZ_CP012830:c2644826-2644596 [Pseudomonas fluorescens]
ATGGAACAGACCACCCTTTTCATGAGCAATCGAAGCCAGGCCGTCCGCTTGCCCAAAGCCGTGGCAATGCCTGAAGACGT
CAAGCGCGTCAACGTTGTAGCCATTGGCCGCGCTCGCATCATCACGCCGGCCGATGAAGCCTGGGATAGCTGGTTCGATG
GCGATAACGTCACTGCCGATTTTATGGCCGACCGCGAGCAACCGGCCGATCAGGAGCGCGAGTCGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N8BYT7

Antitoxin

Source ID Structure
AlphaFold DB A0A1J0EKV4

References