Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 28691..29216 Replicon plasmid pO157-WS4202
Accession NZ_CP012803
Organism Escherichia coli O157:H7 strain WS4202 isolate laboratory strain

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag AO055_RS27325 Protein ID WP_001159868.1
Coordinates 28911..29216 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AO055_RS27320 Protein ID WP_000813634.1
Coordinates 28691..28909 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO055_RS30870 23965..24204 + 240 WP_032195322.1 hypothetical protein -
AO055_RS27285 24325..25065 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
AO055_RS27290 25350..26327 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
AO055_RS27300 26735..26935 - 201 WP_000708307.1 hypothetical protein -
AO055_RS27305 26932..27552 - 621 WP_001248529.1 hypothetical protein -
AO055_RS27310 27549..28232 - 684 WP_010891291.1 DNA-binding protein -
AO055_RS27320 28691..28909 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AO055_RS27325 28911..29216 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
AO055_RS27330 29217..30023 + 807 WP_000016989.1 site-specific integrase -
AO055_RS29865 31921..32259 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
AO055_RS27345 32847..34013 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / toxB / toxB / toxB / toxB / toxB / toxB / toxB / espP 1..92739 92739


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T57247 WP_001159868.1 NZ_CP012803:28911-29216 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T57247 NZ_CP012803:28911-29216 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT57247 WP_000813634.1 NZ_CP012803:28691-28909 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT57247 NZ_CP012803:28691-28909 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References