Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4231826..4232457 Replicon chromosome
Accession NZ_CP012802
Organism Escherichia coli O157:H7 strain WS4202

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag AO055_RS21875 Protein ID WP_001259386.1
Coordinates 4232182..4232457 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AO055_RS21870 Protein ID WP_000593555.1
Coordinates 4231826..4232185 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO055_RS21840 4226938..4227411 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
AO055_RS21845 4227408..4227689 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
AO055_RS21850 4227766..4229124 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
AO055_RS21855 4229117..4230625 + 1509 WP_001301773.1 carbohydrate kinase -
AO055_RS21860 4230615..4230884 + 270 WP_000933060.1 HPr family phosphocarrier protein -
AO055_RS21865 4230916..4231776 + 861 WP_001131788.1 class II aldolase -
AO055_RS21870 4231826..4232185 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AO055_RS21875 4232182..4232457 - 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
AO055_RS21880 4232530..4233654 - 1125 WP_001301659.1 ABC transporter permease -
AO055_RS21885 4233654..4236389 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
AO055_RS21890 4236386..4237453 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T57238 WP_001259386.1 NZ_CP012802:c4232457-4232182 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T57238 NZ_CP012802:c4232457-4232182 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT57238 WP_000593555.1 NZ_CP012802:c4232185-4231826 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT57238 NZ_CP012802:c4232185-4231826 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References