Detailed information of TA system TA057237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4189226..4190026
• Replicon: chromosome
• Accession: NZ_CP012802
• Organism: Escherichia coli O157:H7 strain WS4202

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A891SNC1
• Locus tag: AO055_RS21625
• Protein ID: WP_000342448.1
• Coordinates: 4189226..4189753 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: AO055_RS21630
• Protein ID: WP_001277108.1
• Coordinates: 4189760..4190026 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO055_RS21600	4184301..4185068	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
AO055_RS21605	4185065..4186342	-	1278	WP_000803797.1	branched chain amino acid ABC transporter permease LivM	-
AO055_RS21610	4186339..4187265	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
AO055_RS21615	4187313..4188422	-	1110	WP_001301528.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
AO055_RS21620	4188846..4189229	+	384	WP_000778769.1	aspartate 1-decarboxylase autocleavage activator PanM	-
AO055_RS21625	4189226..4189753	-	528	WP_000342448.1	GNAT family N-acetyltransferase	Toxin
AO055_RS21630	4189760..4190026	-	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
AO055_RS21635	4190176..4191279	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
AO055_RS21640	4191550..4192404	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
AO055_RS21645	4192649..4193707	-	1059	WP_001042018.1	permease-like cell division protein FtsX	-
AO055_RS21650	4193700..4194368	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T57237 WP_000342448.1 NZ_CP012802:c4189753-4189226 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T57237 NZ_CP012802:c4189753-4189226 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT57237 WP_001277108.1 NZ_CP012802:c4190026-4189760 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT57237 NZ_CP012802:c4190026-4189760 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• PDB: 6GTS

Antitoxin

• AlphaFold DB: A0A829CN24