Detailed information of TA system TA057206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 2235009..2235547
• Replicon: chromosome
• Accession: NZ_CP012756
• Organism: Staphylococcus aureus strain JS395

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: -
• Locus tag: ACH32_RS11315
• Protein ID: WP_060649822.1
• Coordinates: 2235221..2235547 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: C5QLS5
• Locus tag: ACH32_RS11310
• Protein ID: WP_001058489.1
• Coordinates: 2235009..2235218 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACH32_RS11275	2231460..2231924	+	465	WP_001085185.1	SsrA-binding protein SmpB	-
ACH32_RS11285	2232487..2233593	-	1107	WP_060649820.1	site-specific integrase	-
ACH32_RS11290	2233599..2234246	-	648	WP_072474260.1	helix-turn-helix domain-containing protein	-
ACH32_RS11295	2234404..2234610	+	207	WP_001060953.1	hypothetical protein	-
ACH32_RS11300	2234646..2234873	+	228	WP_000164237.1	helix-turn-helix domain-containing protein	-
ACH32_RS11305	2234870..2235016	+	147	WP_060649821.1	hypothetical protein	-
ACH32_RS11310	2235009..2235218	+	210	WP_001058489.1	hypothetical protein	Antitoxin
ACH32_RS11315	2235221..2235547	+	327	WP_060649822.1	DUF1474 family protein	Toxin
ACH32_RS11320	2235612..2236481	+	870	WP_060649823.1	primase alpha helix C-terminal domain-containing protein	-
ACH32_RS11325	2236498..2237955	+	1458	WP_060649824.1	virulence-associated E family protein	-
ACH32_RS11330	2238256..2238618	+	363	WP_060649825.1	hypothetical protein	-
ACH32_RS11335	2238620..2238904	+	285	WP_000998182.1	hypothetical protein	-
ACH32_RS11340	2238901..2239542	+	642	WP_001019771.1	hypothetical protein	-
ACH32_RS11345	2239890..2240231	+	342	WP_001190601.1	hypothetical protein	-
ACH32_RS11350	2240245..2240475	+	231	WP_000449693.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2229066..2243321	14255

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 13051.36 Da
• Isoelectric Point: 4.7152

>T57206 WP_060649822.1 NZ_CP012756:2235221-2235547 [Staphylococcus aureus]
MNWEINDLFSDLKLLKDRFEDLKDNHGWHFDNFYPHEPNHVLNKTEAIKEGLSYHERRIHNNQMFDLFHLYIEQFDNIIE
KFHEIEKASSENFGEESDDAKNSIKVAE

Nucleotide

• Length: 327 bp

>T57206 NZ_CP012756:2235221-2235547 [Staphylococcus aureus]
ATGAATTGGGAAATTAATGATTTGTTTAGCGATTTGAAATTGTTGAAAGATAGATTCGAAGATTTAAAGGATAATCATGG
TTGGCATTTTGATAATTTTTATCCTCATGAACCGAATCATGTTTTAAATAAAACCGAAGCGATTAAAGAAGGACTTTCTT
ATCACGAGAGACGTATTCACAATAATCAAATGTTTGATTTATTCCATCTCTATATAGAGCAGTTCGATAATATTATCGAA
AAGTTTCATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGC
AGAGTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8225.24 Da
• Isoelectric Point: 4.2820

>AT57206 WP_001058489.1 NZ_CP012756:2235009-2235218 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVDGIEVVSEVSREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT57206 NZ_CP012756:2235009-2235218 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACGGAATATGTTGATGGTATTGAAGTGGTTTCAGAGGTTAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A853V601