Detailed information of TA system TA057203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 680890..681404
• Replicon: chromosome
• Accession: NZ_CP012756
• Organism: Staphylococcus aureus strain JS395

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: ACH32_RS03660
• Protein ID: WP_060649658.1
• Coordinates: 680890..681099 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: ACH32_RS03665
• Protein ID: WP_000138934.1
• Coordinates: 681111..681404 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACH32_RS03640	676398..677756	-	1359	WP_060649657.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
ACH32_RS03645	677771..678841	-	1071	WP_000159641.1	D-alanine--D-alanine ligase	-
ACH32_RS03650	679159..680361	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
ACH32_RS03655	680625..680762	-	138	WP_000828354.1	hypothetical protein	-
ACH32_RS03660	680890..681099	-	210	WP_060649658.1	heavy-metal-associated domain-containing protein	Toxin
ACH32_RS03665	681111..681404	-	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
ACH32_RS03670	681570..683054	+	1485	WP_000571558.1	cardiolipin synthase	-
ACH32_RS03675	683082..683729	+	648	WP_001187624.1	HD domain-containing protein	-
ACH32_RS03680	684479..685351	-	873	WP_000725802.1	membrane protein insertase YidC	-
ACH32_RS03685	685437..686078	-	642	WP_000483162.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7870.88 Da
• Isoelectric Point: 4.3869

>T57203 WP_060649658.1 NZ_CP012756:c681099-680890 [Staphylococcus aureus]
MIHQNTIYTVGIETKEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T57203 NZ_CP012756:c681099-680890 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGTGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT57203 WP_000138934.1 NZ_CP012756:c681404-681111 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT57203 NZ_CP012756:c681404-681111 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0D1GBN9