Detailed information of TA system TA057202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 661147..661676
• Replicon: chromosome
• Accession: NZ_CP012756
• Organism: Staphylococcus aureus strain JS395

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ACH32_RS03570
• Protein ID: WP_000621175.1
• Coordinates: 661147..661509 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: ACH32_RS03575
• Protein ID: WP_000948331.1
• Coordinates: 661506..661676 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACH32_RS03545	658126..658896	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
ACH32_RS03550	658871..659350	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
ACH32_RS03555	659352..659678	-	327	WP_001052491.1	anti-sigma factor antagonist	-
ACH32_RS03560	659797..660798	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
ACH32_RS03570	661147..661509	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ACH32_RS03575	661506..661676	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
ACH32_RS03580	661761..662909	-	1149	WP_001281140.1	alanine racemase	-
ACH32_RS03585	662975..663334	-	360	WP_000581197.1	holo-ACP synthase	-
ACH32_RS03590	663338..663829	-	492	WP_001205909.1	PH domain-containing protein	-
ACH32_RS03595	663816..665399	-	1584	WP_060649655.1	PH domain-containing protein	-
ACH32_RS03600	665392..665871	-	480	WP_001287077.1	hypothetical protein	-
ACH32_RS03605	666079..666639	-	561	WP_001092421.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T57202 WP_000621175.1 NZ_CP012756:c661509-661147 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T57202 NZ_CP012756:c661509-661147 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT57202 WP_000948331.1 NZ_CP012756:c661676-661506 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT57202 NZ_CP012756:c661676-661506 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1