Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 230410..230932 Replicon plasmid KP-p1
Accession NZ_CP012754
Organism Klebsiella pneumoniae strain KP617

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag AN966_RS27730 Protein ID WP_038991638.1
Coordinates 230410..230694 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag AN966_RS27735 Protein ID WP_004181777.1
Coordinates 230684..230932 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN966_RS29535 225610..226705 + 1096 Protein_235 conjugal transfer protein TraG N-terminal domain-containing protein -
AN966_RS27720 226804..227991 - 1188 WP_043907031.1 transposase -
AN966_RS29540 228027..228455 + 429 WP_077256229.1 IS200/IS605 family transposase -
AN966_RS29545 228534..228758 + 225 Protein_238 transposase -
AN966_RS27725 229030..230256 - 1227 WP_040120327.1 transposase -
AN966_RS31015 230292..230393 + 102 Protein_240 IS200/IS605 family transposase -
AN966_RS27730 230410..230694 - 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AN966_RS27735 230684..230932 - 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AN966_RS27740 231223..233022 + 1800 WP_043907032.1 ATP-dependent helicase -
AN966_RS27745 233356..234342 + 987 WP_040120328.1 hypothetical protein -
AN966_RS27750 234487..234840 + 354 WP_004181774.1 hypothetical protein -
AN966_RS27755 234902..235723 + 822 WP_004181772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-VI / blaNDM-1 / qnrB1 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) - 1..273628 273628


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T57195 WP_038991638.1 NZ_CP012754:c230694-230410 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T57195 NZ_CP012754:c230694-230410 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT57195 WP_004181777.1 NZ_CP012754:c230932-230684 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT57195 NZ_CP012754:c230932-230684 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References