Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3559291..3559916 Replicon chromosome
Accession NZ_CP012753
Organism Klebsiella pneumoniae strain KP617

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag AN966_RS17535 Protein ID WP_002882817.1
Coordinates 3559291..3559674 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AN966_RS17540 Protein ID WP_004150355.1
Coordinates 3559674..3559916 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN966_RS17520 3556657..3557559 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
AN966_RS17525 3557556..3558191 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
AN966_RS17530 3558188..3559117 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AN966_RS17535 3559291..3559674 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
AN966_RS17540 3559674..3559916 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AN966_RS17545 3560121..3561038 + 918 WP_004181612.1 alpha/beta hydrolase -
AN966_RS17550 3561052..3561993 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AN966_RS17555 3562038..3562475 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AN966_RS17560 3562472..3563332 - 861 WP_004146232.1 virulence factor BrkB family protein -
AN966_RS17565 3563326..3563925 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T57190 WP_002882817.1 NZ_CP012753:c3559674-3559291 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T57190 NZ_CP012753:c3559674-3559291 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT57190 WP_004150355.1 NZ_CP012753:c3559916-3559674 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT57190 NZ_CP012753:c3559916-3559674 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References