Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 278798..279483 Replicon plasmid unnamed
Accession NZ_CP012748
Organism Paraburkholderia caribensis MBA4

Toxin (Protein)


Gene name hicA Uniprot ID A0A0P0RLE5
Locus tag K788_RS31595 Protein ID WP_035995604.1
Coordinates 279298..279483 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A4R0X4K9
Locus tag K788_RS31590 Protein ID WP_035995606.1
Coordinates 278798..279217 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K788_RS31570 274260..275588 + 1329 WP_035995615.1 FAD-binding oxidoreductase -
K788_RS31575 275585..276496 + 912 WP_035995613.1 ABC transporter permease -
K788_RS31580 276493..277290 + 798 WP_007583256.1 ABC transporter permease -
K788_RS31585 277311..278738 + 1428 WP_035995609.1 aldehyde dehydrogenase family protein -
K788_RS31590 278798..279217 - 420 WP_035995606.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K788_RS31595 279298..279483 - 186 WP_035995604.1 type II toxin-antitoxin system HicA family toxin Toxin
K788_RS31600 279575..281290 - 1716 WP_035995602.1 GMC family oxidoreductase N-terminal domain-containing protein -
K788_RS31605 281405..282415 - 1011 WP_051453934.1 ABC transporter substrate-binding protein -
K788_RS31610 282666..283010 - 345 WP_035995598.1 DUF861 domain-containing protein -
K788_RS31615 283052..284029 - 978 WP_035995596.1 succinylglutamate desuccinylase/aspartoacylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB / acrB 1..2555069 2555069


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-65)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 7043.11 Da        Isoelectric Point: 11.3397

>T57172 WP_035995604.1 NZ_CP012748:c279483-279298 [Paraburkholderia caribensis MBA4]
MKYSEVRKWLKQQGATFERHRSGSSHYRVTLNGRTTIFPDHGAKEMGKGLVEAIRKQLGIR

Download         Length: 186 bp

>T57172 NZ_CP012748:c279483-279298 [Paraburkholderia caribensis MBA4]
ATGAAATACAGCGAGGTCAGGAAGTGGCTGAAGCAGCAAGGCGCGACGTTCGAAAGGCACCGAAGCGGCTCGAGTCACTA
CCGGGTGACGCTCAATGGCAGGACCACCATCTTCCCCGATCACGGTGCGAAGGAAATGGGTAAAGGACTGGTGGAGGCCA
TCAGAAAGCAACTCGGTATCAGATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15290.69 Da        Isoelectric Point: 4.9658

>AT57172 WP_035995606.1 NZ_CP012748:c279217-278798 [Paraburkholderia caribensis MBA4]
MLSYPISLTPDDNGTLLVTFPDVPEAITVGDDEEDAKVQALDALEAAFEIYFAEKRSIPMPSKAKRGQPVVTLPALVTAK
VLLADEMLRQNVRKAELARRLSVNQVQVDRLLNFGHSSKIEMVESAFAVLGKRLDVRAV

Download         Length: 420 bp

>AT57172 NZ_CP012748:c279217-278798 [Paraburkholderia caribensis MBA4]
ATGCTCAGCTACCCGATCAGCCTGACACCCGACGACAACGGAACGTTGCTCGTCACGTTTCCGGATGTTCCCGAAGCCAT
CACCGTCGGCGACGACGAAGAAGACGCGAAAGTGCAAGCGCTGGATGCGCTCGAGGCCGCGTTCGAAATCTACTTCGCGG
AGAAGCGAAGCATCCCGATGCCGTCAAAGGCCAAACGCGGACAACCCGTCGTCACGCTGCCGGCGCTCGTCACGGCAAAA
GTGCTGCTGGCGGATGAAATGCTCCGCCAGAACGTGCGAAAGGCCGAACTCGCGCGGCGGCTCAGCGTCAATCAGGTGCA
GGTGGATCGACTCCTGAACTTCGGCCATTCATCGAAGATCGAGATGGTCGAGTCGGCGTTTGCGGTATTGGGTAAGCGTC
TTGATGTTCGCGCCGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P0RLE5

Antitoxin

Source ID Structure
AlphaFold DB A0A4R0X4K9

References