Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4251683..4252302 Replicon chromosome
Accession NZ_CP012745
Organism Klebsiella pneumoniae subsp. pneumoniae strain TGH13

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AOG30_RS21185 Protein ID WP_002892050.1
Coordinates 4252084..4252302 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag AOG30_RS21180 Protein ID WP_002892066.1
Coordinates 4251683..4252057 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOG30_RS21170 4246835..4248028 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOG30_RS21175 4248051..4251197 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
AOG30_RS21180 4251683..4252057 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
AOG30_RS21185 4252084..4252302 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AOG30_RS21190 4252465..4253031 + 567 WP_002892030.1 maltose O-acetyltransferase -
AOG30_RS28855 4253003..4253143 - 141 WP_004147370.1 hypothetical protein -
AOG30_RS21200 4253164..4253634 + 471 WP_020802585.1 YlaC family protein -
AOG30_RS21205 4253609..4255060 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
AOG30_RS21210 4255161..4255859 + 699 WP_023287311.1 GNAT family N-acetyltransferase -
AOG30_RS21215 4255856..4255996 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
AOG30_RS21220 4255996..4256259 - 264 WP_032432663.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T57164 WP_002892050.1 NZ_CP012745:4252084-4252302 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T57164 NZ_CP012745:4252084-4252302 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT57164 WP_002892066.1 NZ_CP012745:4251683-4252057 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT57164 NZ_CP012745:4251683-4252057 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References