Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1693941..1694677 Replicon chromosome
Accession NZ_CP012745
Organism Klebsiella pneumoniae subsp. pneumoniae strain TGH13

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag AOG30_RS08430 Protein ID WP_032433360.1
Coordinates 1694195..1694677 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AOG30_RS08425 Protein ID WP_003026799.1
Coordinates 1693941..1694207 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOG30_RS08400 1689587..1690726 + 1140 WP_032433368.1 mannitol dehydrogenase -
AOG30_RS08405 1690755..1691417 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
AOG30_RS08410 1691401..1692405 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
AOG30_RS08415 1692423..1693055 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
AOG30_RS08420 1693065..1693628 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
AOG30_RS08425 1693941..1694207 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AOG30_RS08430 1694195..1694677 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
AOG30_RS08435 1695038..1695637 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
AOG30_RS08440 1695850..1696794 - 945 WP_077254249.1 fimbrial protein -
AOG30_RS08445 1696806..1697384 - 579 WP_032432061.1 type 1 fimbrial protein -
AOG30_RS08450 1697388..1698128 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T57154 WP_032433360.1 NZ_CP012745:1694195-1694677 [Klebsiella pneumoniae subsp. pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T57154 NZ_CP012745:1694195-1694677 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT57154 WP_003026799.1 NZ_CP012745:1693941-1694207 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT57154 NZ_CP012745:1693941-1694207 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References