Detailed information of TA system TA057133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5361370..5361995
• Replicon: chromosome
• Accession: NZ_CP012743
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain TGH8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AOD72_RS26800
• Protein ID: WP_062921072.1
• Coordinates: 5361370..5361753 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: AOD72_RS26805
• Protein ID: WP_004150355.1
• Coordinates: 5361753..5361995 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOD72_RS26785	5358736..5359638	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
AOD72_RS26790	5359635..5360270	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
AOD72_RS26795	5360267..5361196	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
AOD72_RS26800	5361370..5361753	-	384	WP_062921072.1	type II toxin-antitoxin system VapC family toxin	Toxin
AOD72_RS26805	5361753..5361995	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
AOD72_RS26810	5362200..5363117	+	918	WP_062921073.1	alpha/beta hydrolase	-
AOD72_RS26815	5363131..5364072	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
AOD72_RS26820	5364117..5364554	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AOD72_RS26825	5364551..5365411	-	861	WP_004146232.1	virulence factor BrkB family protein	-
AOD72_RS26830	5365405..5366004	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T57133 WP_062921072.1 NZ_CP012743:c5361753-5361370 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDALILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T57133 NZ_CP012743:c5361753-5361370 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCCTCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT57133 WP_004150355.1 NZ_CP012743:c5361995-5361753 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT57133 NZ_CP012743:c5361995-5361753 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9