Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4142626..4143245 Replicon chromosome
Accession NZ_CP012743
Organism Klebsiella pneumoniae subsp. pneumoniae strain TGH8

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AOD72_RS20730 Protein ID WP_002892050.1
Coordinates 4143027..4143245 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag AOD72_RS20725 Protein ID WP_002892066.1
Coordinates 4142626..4143000 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOD72_RS20715 4137778..4138971 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOD72_RS20720 4138994..4142140 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
AOD72_RS20725 4142626..4143000 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
AOD72_RS20730 4143027..4143245 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AOD72_RS20735 4143404..4143970 + 567 WP_002892030.1 maltose O-acetyltransferase -
AOD72_RS30690 4143942..4144082 - 141 WP_004147370.1 hypothetical protein -
AOD72_RS20745 4144103..4144573 + 471 WP_002892026.1 YlaC family protein -
AOD72_RS20750 4144548..4145999 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
AOD72_RS20755 4146100..4146798 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
AOD72_RS20760 4146795..4146935 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
AOD72_RS20765 4146935..4147198 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T57130 WP_002892050.1 NZ_CP012743:4143027-4143245 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T57130 NZ_CP012743:4143027-4143245 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT57130 WP_002892066.1 NZ_CP012743:4142626-4143000 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT57130 NZ_CP012743:4142626-4143000 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References