Detailed information of TA system TA057117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4054..4725
• Replicon: plasmid unnamed
• Accession: NZ_CP012741
• Organism: Vibrio vulnificus strain FORC_017

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: FORC17_RS23155
• Protein ID: WP_011149008.1
• Coordinates: 4054..4353 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: Q7MBL1
• Locus tag: FORC17_RS23160
• Protein ID: WP_011149007.1
• Coordinates: 4429..4725 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC17_RS23110	44..427	+	384	WP_065091372.1	hypothetical protein	-
FORC17_RS25260	439..603	+	165	WP_158513833.1	hypothetical protein	-
FORC17_RS23115	614..910	+	297	WP_065091373.1	hypothetical protein	-
FORC17_RS23120	924..1151	+	228	WP_011988355.1	hypothetical protein	-
FORC17_RS23125	1189..1527	+	339	WP_065091374.1	hypothetical protein	-
FORC17_RS23130	1534..1818	+	285	WP_065091375.1	hypothetical protein	-
FORC17_RS23135	2359..2862	+	504	WP_011149015.1	hypothetical protein	-
FORC17_RS23140	2865..3173	+	309	WP_065091376.1	DUF1738 domain-containing protein	-
FORC17_RS23145	3240..3563	+	324	WP_065091377.1	type I restriction endonuclease subunit M	-
FORC17_RS23150	3798..4016	+	219	WP_065091378.1	hypothetical protein	-
FORC17_RS23155	4054..4353	+	300	WP_011149008.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC17_RS23160	4429..4725	+	297	WP_011149007.1	putative addiction module antidote protein	Antitoxin
FORC17_RS23165	5301..6683	+	1383	WP_065091379.1	DUF3560 domain-containing protein	-
FORC17_RS23170	6765..7211	+	447	WP_065091380.1	lytic transglycosylase domain-containing protein	-
FORC17_RS23175	7769..8128	+	360	WP_011149002.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..70069	70069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-92)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11134.96 Da
• Isoelectric Point: 10.6229

>T57117 WP_011149008.1 NZ_CP012741:4054-4353 [Vibrio vulnificus]
MIYNIKRTEIFNDWLRELKDPQARGSIAARVQRLTQGLNGDVKPVGAGISELRIHVGKGYRVYFKRSGKQIIVLLCGGNK
KTQNQDIELAKQLAQELGL

Nucleotide

• Length: 300 bp

>T57117 NZ_CP012741:4054-4353 [Vibrio vulnificus]
ATGATATATAATATCAAGCGCACCGAAATCTTTAATGATTGGCTGAGGGAACTAAAAGACCCTCAAGCGCGAGGTTCAAT
CGCCGCGCGAGTGCAACGACTTACCCAAGGATTGAACGGAGACGTGAAACCTGTTGGTGCTGGTATCAGTGAACTCAGGA
TACATGTCGGAAAGGGTTACAGGGTTTACTTTAAGCGAAGCGGCAAGCAGATCATTGTTTTGTTGTGTGGCGGTAACAAG
AAAACTCAAAACCAAGACATTGAACTAGCAAAGCAGCTTGCTCAAGAACTTGGACTGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10437.90 Da
• Isoelectric Point: 4.6855

>AT57117 WP_011149007.1 NZ_CP012741:4429-4725 [Vibrio vulnificus]
MSKNTAMLADFDPAEYLDTPEAIQAYLDEAVASGDAAFISQSLGVIARAKGMTELAEQTGLSREALYRTLSQKGNPNFKG
FMAIISALGISLKLNVHA

Nucleotide

• Length: 297 bp

>AT57117 NZ_CP012741:4429-4725 [Vibrio vulnificus]
ATGTCTAAAAATACAGCTATGCTCGCAGATTTTGACCCTGCTGAGTATCTTGATACCCCAGAAGCTATACAGGCGTATCT
CGATGAAGCTGTAGCCTCTGGTGATGCAGCTTTTATCAGTCAATCTCTTGGTGTGATTGCTCGCGCTAAGGGTATGACAG
AACTTGCAGAACAAACAGGATTATCCCGAGAAGCGCTATATAGAACGCTTAGCCAAAAGGGAAACCCTAATTTTAAAGGA
TTTATGGCCATCATTTCTGCTTTGGGCATCTCTTTGAAATTGAACGTTCATGCTTAA

Structures

Antitoxin

• AlphaFold DB: Q7MBL1