Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1963602..1964256 Replicon chromosome
Accession NZ_CP012735
Organism Shigella flexneri 1a strain 0228

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag AOT98_RS10980 Protein ID WP_000244767.1
Coordinates 1963849..1964256 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D2A730
Locus tag AOT98_RS10975 Protein ID WP_000354052.1
Coordinates 1963602..1963868 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOT98_RS10950 (1958772) 1958772..1959515 + 744 WP_000951957.1 SDR family oxidoreductase -
AOT98_RS10955 (1959572) 1959572..1961005 - 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
AOT98_RS10960 (1961050) 1961050..1961360 + 311 Protein_1874 N(4)-acetylcytidine aminohydrolase -
AOT98_RS10965 (1961524) 1961524..1962183 + 660 WP_000250274.1 hemolysin III family protein -
AOT98_RS10970 (1962379) 1962379..1963359 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOT98_RS10975 (1963602) 1963602..1963868 + 267 WP_000354052.1 FAD assembly factor SdhE Antitoxin
AOT98_RS10980 (1963849) 1963849..1964256 + 408 WP_000244767.1 protein YgfX Toxin
AOT98_RS10985 (1964296) 1964296..1964817 - 522 WP_001055867.1 flavodoxin FldB -
AOT98_RS10990 (1964929) 1964929..1965825 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
AOT98_RS10995 (1965850) 1965850..1966560 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOT98_RS11000 (1966566) 1966566..1968299 + 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T57090 WP_000244767.1 NZ_CP012735:1963849-1964256 [Shigella flexneri 1a]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T57090 NZ_CP012735:1963849-1964256 [Shigella flexneri 1a]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10519.05 Da        Isoelectric Point: 5.1599

>AT57090 WP_000354052.1 NZ_CP012735:1963602-1963868 [Shigella flexneri 1a]
MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT57090 NZ_CP012735:1963602-1963868 [Shigella flexneri 1a]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGTCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB D2A730

References