Detailed information of TA system TA057085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 44808..45330
• Replicon: plasmid unnamed
• Accession: NZ_CP012734
• Organism: Shigella flexneri 1a strain 0228

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: AOT98_RS00730
• Protein ID: WP_000220560.1
• Coordinates: 45049..45330 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: AOT98_RS00725
• Protein ID: WP_000121743.1
• Coordinates: 44808..45059 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOT98_RS00695 (AOT98_25440)	39888..40550	+	663	WP_000516402.1	division plane positioning ATPase MipZ	-
AOT98_RS00700 (AOT98_25445)	40648..40929	+	282	WP_001185482.1	hypothetical protein	-
AOT98_RS00705 (AOT98_25450)	40954..41931	+	978	WP_024127745.1	hypothetical protein	-
AOT98_RS00710 (AOT98_25455)	41928..42284	-	357	WP_052921318.1	DNA distortion polypeptide 3	-
AOT98_RS00715 (AOT98_25460)	42421..42867	-	447	WP_001074386.1	hypothetical protein	-
AOT98_RS00720 (AOT98_25465)	42892..43746	-	855	WP_001239324.1	replication initiation protein	-
AOT98_RS00725 (AOT98_25470)	44808..45059	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
AOT98_RS00730 (AOT98_25475)	45049..45330	+	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOT98_RS00735 (AOT98_25480)	45476..45799	+	324	WP_001181903.1	hypothetical protein	-
AOT98_RS00740 (AOT98_25485)	45844..46089	+	246	WP_001550454.1	hypothetical protein	-
AOT98_RS00745 (AOT98_25490)	46079..46294	+	216	WP_052921379.1	hypothetical protein	-
AOT98_RS00750 (AOT98_25495)	46387..46716	+	330	WP_000866648.1	hypothetical protein	-
AOT98_RS00755 (AOT98_25500)	46759..46938	+	180	WP_000439078.1	hypothetical protein	-
AOT98_RS29575	47008..47157	+	150	WP_000003880.1	hypothetical protein	-
AOT98_RS26130	47170..47442	+	273	WP_000160399.1	hypothetical protein	-
AOT98_RS00765 (AOT98_25510)	47768..48313	+	546	WP_000757693.1	DNA distortion polypeptide 1	-
AOT98_RS00770 (AOT98_25515)	48316..49482	+	1167	WP_000539529.1	MobP1 family relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS1 / blaTEM-1B	-	1..63412	63412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T57085 WP_000220560.1 NZ_CP012734:45049-45330 [Shigella flexneri 1a]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T57085 NZ_CP012734:45049-45330 [Shigella flexneri 1a]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT57085 WP_000121743.1 NZ_CP012734:44808-45059 [Shigella flexneri 1a]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT57085 NZ_CP012734:44808-45059 [Shigella flexneri 1a]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301