Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 241201..241843 Replicon chromosome
Accession NZ_CP012728
Organism Bacillus anthracis strain PR10-4

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag AOD69_RS01375 Protein ID WP_000635963.1
Coordinates 241493..241843 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag AOD69_RS01370 Protein ID WP_000004570.1
Coordinates 241201..241488 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOD69_RS01345 236517..237479 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
AOD69_RS01350 237472..238044 - 573 WP_000906916.1 rhomboid family intramembrane serine protease -
AOD69_RS01355 238137..238496 + 360 WP_000635040.1 holo-ACP synthase -
AOD69_RS01360 238653..239603 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
AOD69_RS01365 239722..240891 + 1170 WP_000390596.1 alanine racemase -
AOD69_RS01370 241201..241488 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
AOD69_RS01375 241493..241843 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
AOD69_RS01380 241911..244079 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
AOD69_RS01385 244137..244253 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
AOD69_RS01390 244449..244907 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T57071 WP_000635963.1 NZ_CP012728:241493-241843 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T57071 NZ_CP012728:241493-241843 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT57071 WP_000004570.1 NZ_CP012728:241201-241488 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT57071 NZ_CP012728:241201-241488 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References