Detailed information of TA system TA057043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parE_1-relB/RelE(toxin)
• Location: 4240555..4241009
• Replicon: chromosome
• Accession: NZ_CP012700
• Organism: Sphingopyxis macrogoltabida strain EY-1

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0N7GT50
• Locus tag: AN936_RS19645
• Protein ID: WP_054589566.1
• Coordinates: 4240555..4240857 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AN936_RS25335
• Protein ID: WP_201783006.1
• Coordinates: 4240854..4241009 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN936_RS19620	4235815..4236849	-	1035	WP_054589563.1	patatin-like phospholipase family protein	-
AN936_RS24295	4236846..4237295	-	450	WP_201783003.1	hypothetical protein	-
AN936_RS19625	4237298..4238530	-	1233	WP_201783004.1	nucleotidyltransferase	-
AN936_RS19630	4238733..4239053	+	321	WP_054589564.1	helix-turn-helix domain-containing protein	-
AN936_RS19635	4239104..4239973	+	870	WP_201783005.1	ImmA/IrrE family metallo-endopeptidase	-
AN936_RS19640	4239985..4240473	-	489	WP_201782942.1	hypothetical protein	-
AN936_RS19645	4240555..4240857	-	303	WP_054589566.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AN936_RS25335	4240854..4241009	-	156	WP_201783006.1	stability determinant	Antitoxin
AN936_RS19655	4241494..4242177	+	684	WP_054589567.1	recombinase family protein	-
AN936_RS25150	4242201..4242314	-	114	WP_158500121.1	antitoxin VbhA family protein	-
AN936_RS24820	4242589..4242678	-	90	Protein_3957	DUF454 domain-containing protein	-
AN936_RS19665	4242843..4243745	-	903	WP_054589569.1	SDR family oxidoreductase	-
AN936_RS19670	4244224..4245579	-	1356	WP_084758549.1	IS1380-like element IS1247 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4178073..4312084	134011
	-	flank	IS/Tn	-	-	4244224..4245579	1355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11725.74 Da
• Isoelectric Point: 11.0233

>T57043 WP_054589566.1 NZ_CP012700:c4240857-4240555 [Sphingopyxis macrogoltabida]
MTAKPYRIEWRPMAREDLRGIVRYIGKDNPTRAKSFGKELRDKTKPLAQHPELGRKGRPGLPEWLRELVVHPNYIIFYRV
LAEARTVEILRVKHAAQQVP

Nucleotide

• Length: 303 bp

>T57043 NZ_CP012700:c4240857-4240555 [Sphingopyxis macrogoltabida]
ATGACGGCCAAGCCTTACCGCATTGAGTGGCGGCCTATGGCCCGCGAGGATTTGCGCGGCATCGTGCGCTACATCGGCAA
GGACAACCCGACGCGGGCCAAGAGCTTCGGCAAGGAATTGCGCGACAAGACCAAGCCGCTTGCACAGCATCCAGAGCTTG
GCCGCAAAGGGCGGCCGGGCTTGCCCGAGTGGCTGCGCGAACTGGTGGTGCATCCCAACTACATCATTTTTTATCGCGTG
CTGGCCGAGGCCCGCACCGTCGAGATTTTGAGAGTGAAGCACGCGGCCCAACAGGTGCCGTGA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5614.33 Da
• Isoelectric Point: 4.8990

>AT57043 WP_201783006.1 NZ_CP012700:c4241009-4240854 [Sphingopyxis macrogoltabida]
MRGAHEAAAYNQWLAGEIQASIDDPRPNIPHDEVMAEMDADIAALPKKKRA

Nucleotide

• Length: 156 bp

>AT57043 NZ_CP012700:c4241009-4240854 [Sphingopyxis macrogoltabida]
ATGCGCGGCGCACATGAAGCCGCCGCCTATAACCAGTGGCTGGCCGGCGAGATTCAGGCGTCCATTGACGACCCCCGGCC
GAACATCCCTCACGATGAAGTCATGGCCGAGATGGACGCCGACATTGCCGCCTTGCCGAAGAAAAAGCGCGCATGA

Structures

Toxin

• AlphaFold DB: A0A0N7GT50