Detailed information of TA system TA057042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 3889486..3890113
• Replicon: chromosome
• Accession: NZ_CP012700
• Organism: Sphingopyxis macrogoltabida strain EY-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AN936_RS18140
• Protein ID: WP_054589307.1
• Coordinates: 3889486..3889878 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0N7I258
• Locus tag: AN936_RS18145
• Protein ID: WP_054589308.1
• Coordinates: 3889883..3890113 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN936_RS18115	3886049..3886525	+	477	WP_011542707.1	nuclear transport factor 2 family protein	-
AN936_RS18120	3886754..3887383	+	630	WP_037553289.1	cytochrome c	-
AN936_RS18125	3887569..3887853	+	285	Protein_3644	type II restriction endonuclease	-
AN936_RS18130	3888031..3888351	-	321	Protein_3645	ATP-binding protein	-
AN936_RS18135	3888520..3888978	-	459	WP_054589306.1	MucR family transcriptional regulator	-
AN936_RS24785	3889125..3889328	-	204	WP_149037709.1	hypothetical protein	-
AN936_RS18140	3889486..3889878	-	393	WP_054589307.1	PIN domain-containing protein	Toxin
AN936_RS18145	3889883..3890113	-	231	WP_054589308.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AN936_RS25130	3891187..3891333	-	147	WP_158500115.1	hypothetical protein	-
AN936_RS18150	3891484..3891888	-	405	WP_054589309.1	helix-turn-helix domain-containing protein	-
AN936_RS24790	3892101..3892469	-	369	WP_149037710.1	hypothetical protein	-
AN936_RS24795	3892895..3893554	-	660	WP_149037711.1	hypothetical protein	-
AN936_RS18160	3893801..3894463	-	663	WP_054589311.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3888031..3888348	317

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-113)

Antitoxin

• SpoVT-AbrB domain (5-32)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14238.17 Da
• Isoelectric Point: 4.2178

>T57042 WP_054589307.1 NZ_CP012700:c3889878-3889486 [Sphingopyxis macrogoltabida]
MPVFLDTNILIYAFSDDPRTSTAEKLLSDGGEISVQVLNEFSNVARKKLQFDWQQLGEALGIVRGLASRVNPLNVETHIE
GIALAERYGISIYDGLIVASAIQAGCDILYSEDMHDGLGIGEQLRIVNPF

Nucleotide

• Length: 393 bp

>T57042 NZ_CP012700:c3889878-3889486 [Sphingopyxis macrogoltabida]
ATGCCCGTCTTTCTCGATACGAATATCCTCATCTACGCGTTCAGCGACGATCCGCGCACCAGCACAGCTGAGAAACTCTT
GTCGGACGGGGGCGAAATCAGCGTTCAAGTTCTGAACGAATTCTCGAACGTCGCCCGGAAGAAACTTCAGTTCGATTGGC
AACAGCTCGGCGAAGCGCTGGGTATCGTTCGGGGTCTCGCAAGCCGCGTTAATCCCCTCAACGTAGAAACCCATATCGAG
GGGATCGCACTAGCGGAGCGATACGGGATCTCAATTTATGATGGGCTTATCGTTGCGTCCGCAATTCAAGCGGGATGCGA
CATACTATATTCGGAAGACATGCATGACGGACTGGGAATCGGCGAACAGTTGAGGATCGTGAATCCGTTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8887.18 Da
• Isoelectric Point: 8.9114

>AT57042 WP_054589308.1 NZ_CP012700:c3890113-3889883 [Sphingopyxis macrogoltabida]
MQLARWGNSLALRIPASIVELLGLKEGDDIEVNVAGTHRFEIDRDRSREKLVERMRMLRKPLPAGWRFDRDDANER

Nucleotide

• Length: 231 bp

>AT57042 NZ_CP012700:c3890113-3889883 [Sphingopyxis macrogoltabida]
ATGCAGCTCGCGCGCTGGGGAAATAGCCTCGCCCTTCGGATACCGGCATCCATCGTCGAATTGCTTGGTCTCAAGGAAGG
CGACGATATTGAGGTCAATGTCGCCGGGACTCACCGTTTCGAGATCGATCGGGATAGGTCTCGCGAGAAGCTGGTGGAAC
GCATGCGCATGTTGCGCAAGCCTTTGCCTGCCGGTTGGCGCTTTGATCGCGACGACGCCAACGAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0N7I258