Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2026345..2026934 Replicon chromosome
Accession NZ_CP012700
Organism Sphingopyxis macrogoltabida strain EY-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A0N9UXS6
Locus tag AN936_RS09600 Protein ID WP_054587954.1
Coordinates 2026345..2026713 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0N9UYJ4
Locus tag AN936_RS09605 Protein ID WP_054587955.1
Coordinates 2026710..2026934 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN936_RS09580 2021523..2022401 - 879 WP_054587950.1 dienelactone hydrolase family protein -
AN936_RS09585 2022828..2023529 - 702 WP_054587951.1 HAD family phosphatase -
AN936_RS09590 2023566..2024483 - 918 WP_149037638.1 hypothetical protein -
AN936_RS09595 2024490..2026313 - 1824 WP_054587953.1 glutamine--fructose-6-phosphate transaminase (isomerizing) -
AN936_RS09600 2026345..2026713 - 369 WP_054587954.1 PIN domain-containing protein Toxin
AN936_RS09605 2026710..2026934 - 225 WP_054587955.1 ribbon-helix-helix domain-containing protein Antitoxin
AN936_RS09610 2027107..2027424 - 318 WP_054587956.1 hypothetical protein -
AN936_RS09615 2027421..2027966 - 546 WP_054587957.1 GNAT family N-acetyltransferase -
AN936_RS09620 2027971..2029341 - 1371 WP_054587958.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
AN936_RS09625 2029389..2030051 + 663 WP_054587959.1 HAD-IA family hydrolase -
AN936_RS09630 2030048..2031061 - 1014 WP_054590206.1 nucleotidyltransferase family protein -
AN936_RS09635 2031058..2031924 - 867 WP_054587960.1 HprK-related kinase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-105)

Antitoxin

Ribbon-helix-helix protein, CopG

(7-38)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13080.16 Da        Isoelectric Point: 9.1636

>T57041 WP_054587954.1 NZ_CP012700:c2026713-2026345 [Sphingopyxis macrogoltabida]
MIEAQFDSDILIDALNGVEGARSEIRRAGRKSVSRISWTEVMSAADPASVKAVEAFLGCFAIEEVGDAVARRAAALRAER
KGLTMADAFVLATAQISGRILVTRNIKVFPASMPGIRVPYTL

Download         Length: 369 bp

>T57041 NZ_CP012700:c2026713-2026345 [Sphingopyxis macrogoltabida]
ATGATCGAGGCACAATTCGACAGCGATATCCTCATCGACGCGCTGAACGGCGTCGAGGGCGCGCGCAGCGAGATCCGCCG
CGCCGGACGCAAGAGCGTCAGCCGGATCAGCTGGACCGAGGTGATGTCGGCCGCCGACCCAGCGTCGGTGAAGGCGGTCG
AGGCCTTTCTCGGCTGTTTCGCGATCGAGGAGGTCGGCGATGCGGTGGCCCGACGCGCTGCTGCGTTGAGGGCCGAACGC
AAGGGGTTGACGATGGCCGACGCCTTCGTCTTAGCAACCGCGCAAATATCGGGCCGCATCCTCGTTACGCGCAATATCAA
GGTGTTTCCGGCATCGATGCCGGGTATTCGCGTTCCCTACACGCTATAG

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8424.48 Da        Isoelectric Point: 6.2939

>AT57041 WP_054587955.1 NZ_CP012700:c2026934-2026710 [Sphingopyxis macrogoltabida]
MRFLADIPDDDVQWLDALAASQGVSRAELMRRAVSAYRADISGDAIDNAFGIWRLRDDIGDGLKYQRRLRGKRE

Download         Length: 225 bp

>AT57041 NZ_CP012700:c2026934-2026710 [Sphingopyxis macrogoltabida]
ATGCGCTTCCTCGCCGATATTCCCGATGACGATGTCCAGTGGCTCGATGCGCTCGCGGCGTCGCAGGGCGTGTCGCGCGC
CGAACTGATGCGCCGCGCGGTGAGCGCGTACCGCGCCGACATATCGGGCGACGCGATCGACAATGCGTTCGGGATATGGC
GTTTGCGTGACGATATCGGCGACGGGCTCAAATATCAGCGCCGCCTCAGGGGCAAGCGCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0N9UXS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0N9UYJ4

References