Detailed information of TA system TA057039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 458035..459506
• Replicon: chromosome
• Accession: NZ_CP012700
• Organism: Sphingopyxis macrogoltabida strain EY-1

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: A0A246JNY4
• Locus tag: AN936_RS02230
• Protein ID: WP_054586720.1
• Coordinates: 458250..459506 (+)
• Length: 419 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: AN936_RS02225
• Protein ID: WP_054586719.1
• Coordinates: 458035..458253 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN936_RS02210	453898..454299	+	402	WP_054586717.1	DUF2958 domain-containing protein	-
AN936_RS02215	454418..456550	+	2133	WP_084758123.1	ParB/RepB/Spo0J family partition protein	-
AN936_RS02220	456751..457779	+	1029	WP_054586718.1	toprim domain-containing protein	-
AN936_RS02225	458035..458253	+	219	WP_054586719.1	helix-turn-helix transcriptional regulator	Antitoxin
AN936_RS02230	458250..459506	+	1257	WP_054586720.1	type II toxin-antitoxin system HipA family toxin	Toxin
AN936_RS02235	459587..459778	+	192	WP_158213708.1	hypothetical protein	-
AN936_RS02240	460058..460999	+	942	WP_054586722.1	DUF2493 domain-containing protein	-
AN936_RS02245	461065..461988	+	924	WP_054586723.1	nucleotidyltransferase and HEPN domain-containing protein	-
AN936_RS02250	461985..462824	+	840	WP_054586724.1	NYN domain-containing protein	-
AN936_RS02255	463569..463967	+	399	WP_054586725.1	DUF3703 domain-containing protein	-
AN936_RS02260	463964..464350	+	387	WP_149037572.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	450298..528677	78379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (146-377)

Antitoxin

• Transcription regulator, HipB-like (6-59)

Sequences

Toxin

Protein

• Length: 419 a.a.
• Molecular weight: 44692.98 Da
• Isoelectric Point: 4.6526

>T57039 WP_054586720.1 NZ_CP012700:458250-459506 [Sphingopyxis macrogoltabida]
MSVLIIYWDGVRVGNLARNGEGTMLFRYDDEWLADPAFRSISASLPRQEGAFTRAQTRPFFAGLLPDEGQREAAARAAGV
SRQNDYALLDALGGDVAGALVILPEAEAVPAPSAAAPEALDQDALAALLEELPRRPFLVGDEGIRMSLAGAQPKLPVILV
DGAPALPPPGQPTTHIVKPAIARFENSVETEAFAMRLAAAVDLDVARVEPRRAGAIAYLLVERFDRRLEGGIIRRIHQED
FCQALGIAPENKYAIEGGPGFAACFDLVRRTCTTPARDLLRLLDAAIFNAVIGNADAHGKNFSLLYADDEGDAATHLAPL
YDLMVTAYYPEVATRFAMPIGRANQLENLDADAWARFAADMQLGVPFVRRRVYTLAGRIRDAAPAVAATLAAEGCEASVL
DNLAALVAARADRIGGAL

Nucleotide

• Length: 1257 bp

>T57039 NZ_CP012700:458250-459506 [Sphingopyxis macrogoltabida]
ATGAGTGTCCTCATCATTTATTGGGATGGCGTGCGGGTCGGCAATCTGGCCCGCAATGGCGAGGGCACCATGTTGTTCCG
CTATGACGACGAATGGCTCGCCGATCCGGCGTTTCGCTCCATTTCGGCATCGCTTCCCCGGCAAGAGGGGGCCTTTACCC
GCGCCCAAACCCGGCCCTTTTTCGCTGGCCTTCTCCCCGACGAGGGGCAGCGCGAGGCCGCCGCCCGGGCCGCGGGCGTC
TCGCGGCAGAATGACTATGCGCTGCTCGACGCCCTTGGCGGCGATGTCGCCGGTGCGCTCGTCATTCTGCCGGAAGCGGA
GGCGGTGCCCGCGCCGTCGGCCGCGGCACCCGAAGCTCTCGATCAGGACGCGCTGGCGGCGCTTCTGGAAGAGCTTCCGC
GCCGTCCCTTTCTCGTCGGCGACGAGGGCATCCGCATGTCGCTCGCCGGCGCGCAGCCGAAGCTTCCCGTCATATTGGTC
GATGGCGCGCCGGCCTTGCCGCCGCCGGGGCAGCCGACGACACATATCGTCAAGCCCGCGATCGCCCGCTTCGAGAATAG
CGTCGAAACCGAGGCCTTCGCGATGCGTCTCGCGGCGGCGGTCGATCTTGACGTTGCCCGGGTCGAGCCGCGGCGGGCGG
GCGCGATCGCCTACCTCCTCGTCGAACGCTTCGACCGGCGCCTTGAGGGCGGCATCATTCGCCGTATCCACCAGGAAGAC
TTCTGCCAGGCGCTCGGTATCGCGCCCGAGAATAAATATGCGATCGAGGGCGGGCCGGGCTTTGCCGCCTGCTTCGACCT
CGTCCGCCGGACCTGCACGACGCCCGCGCGCGACCTGCTGCGTCTGCTCGACGCCGCCATCTTCAATGCGGTCATCGGCA
ACGCCGACGCGCATGGCAAGAATTTCAGCCTTCTCTACGCAGATGATGAAGGCGACGCGGCGACGCATCTGGCACCGCTC
TACGACCTGATGGTCACCGCTTATTATCCCGAAGTGGCGACCCGCTTCGCCATGCCCATCGGACGCGCGAACCAGCTCGA
AAATCTCGATGCGGACGCATGGGCGCGCTTCGCTGCCGACATGCAGCTTGGCGTCCCCTTCGTCCGCCGCCGCGTATACA
CACTCGCAGGCCGTATACGCGACGCTGCGCCCGCTGTTGCCGCGACCCTTGCCGCAGAGGGCTGCGAAGCGTCGGTGCTG
GACAACCTCGCCGCACTCGTCGCCGCGCGCGCCGACAGGATCGGCGGCGCGCTCTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 7551.89 Da
• Isoelectric Point: 10.9746

>AT57039 WP_054586719.1 NZ_CP012700:458035-458253 [Sphingopyxis macrogoltabida]
MDIGSIGQMVRRVRKRAGLRQDELAGAAGVGIRFIVDLEAGKPTLQVGKVLHVLDALGIVVALTTRSEGLTG

Nucleotide

• Length: 219 bp

>AT57039 NZ_CP012700:458035-458253 [Sphingopyxis macrogoltabida]
ATGGATATCGGTAGCATAGGGCAGATGGTGCGCCGCGTGCGCAAGCGGGCAGGGCTCCGCCAAGACGAACTCGCGGGCGC
GGCGGGCGTCGGCATCCGCTTCATCGTCGATCTCGAAGCCGGCAAGCCGACTCTCCAGGTCGGCAAGGTGCTGCATGTCC
TCGACGCGCTCGGCATCGTCGTTGCGCTGACCACGCGTAGCGAAGGCCTGACGGGATGA

Structures

Toxin

• AlphaFold DB: A0A246JNY4