Detailed information of TA system TA057027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3246745..3247271
• Replicon: chromosome
• Accession: NZ_CP012693
• Organism: Escherichia coli strain FORC_028

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: FORC28_RS17000
• Protein ID: WP_000323025.1
• Coordinates: 3246745..3247032 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: FORC28_RS17005
• Protein ID: WP_000534858.1
• Coordinates: 3247032..3247271 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC28_RS16965	3242474..3243523	-	1050	WP_000611215.1	site-specific DNA-methyltransferase	-
FORC28_RS33300	3243674..3243871	-	198	WP_000917767.1	hypothetical protein	-
FORC28_RS16975	3244103..3244645	-	543	WP_000640017.1	DUF1133 family protein	-
FORC28_RS16980	3244654..3245019	-	366	WP_000140024.1	RusA family crossover junction endodeoxyribonuclease	-
FORC28_RS16985	3245020..3246075	-	1056	WP_001369253.1	DUF968 domain-containing protein	-
FORC28_RS30925	3246077..3246349	-	273	WP_012817871.1	hypothetical protein	-
FORC28_RS16995	3246517..3246672	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
FORC28_RS17000	3246745..3247032	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
FORC28_RS17005	3247032..3247271	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
FORC28_RS30930	3247296..3247601	+	306	WP_001326990.1	hypothetical protein	-
FORC28_RS17010	3247804..3248136	+	333	WP_001317460.1	FlxA-like family protein	-
FORC28_RS17015	3248573..3249913	-	1341	WP_000589012.1	ISNCY family transposase	-
FORC28_RS17020	3249947..3250366	-	420	WP_001151195.1	DUF977 family protein	-
FORC28_RS17025	3250407..3251372	-	966	WP_000054507.1	hypothetical protein	-
FORC28_RS17030	3251353..3251874	-	522	WP_000705349.1	hypothetical protein	-
FORC28_RS17035	3251858..3252085	-	228	WP_000476993.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / nleA/espI	3204163..3297476	93313

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T57027 WP_000323025.1 NZ_CP012693:c3247032-3246745 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T57027 NZ_CP012693:c3247032-3246745 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT57027 WP_000534858.1 NZ_CP012693:c3247271-3247032 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT57027 NZ_CP012693:c3247271-3247032 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8