Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2861915..2862441 Replicon chromosome
Accession NZ_CP012693
Organism Escherichia coli strain FORC_028

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FORC28_RS15085 Protein ID WP_000323025.1
Coordinates 2862154..2862441 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FORC28_RS15080 Protein ID WP_000534858.1
Coordinates 2861915..2862154 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC28_RS15060 2857004..2857582 + 579 WP_001204666.1 sce7726 family protein -
FORC28_RS15065 2857542..2858639 - 1098 WP_000156210.1 beta family protein -
FORC28_RS15070 2859273..2860613 + 1341 WP_000589012.1 ISNCY family transposase -
FORC28_RS15075 2861050..2861382 - 333 WP_001317460.1 FlxA-like family protein -
FORC28_RS30750 2861585..2861890 - 306 WP_001326990.1 hypothetical protein -
FORC28_RS15080 2861915..2862154 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FORC28_RS15085 2862154..2862441 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FORC28_RS15090 2862513..2862668 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FORC28_RS15095 2862885..2863136 + 252 WP_000980994.1 hypothetical protein -
FORC28_RS15100 2863203..2863481 + 279 WP_012304870.1 hypothetical protein -
FORC28_RS15105 2863483..2864532 + 1050 WP_001265198.1 DUF968 domain-containing protein -
FORC28_RS15110 2864546..2865298 + 753 WP_001047133.1 antitermination protein -
FORC28_RS33235 2865576..2865665 - 90 WP_120795389.1 hypothetical protein -
FORC28_RS15115 2865720..2865932 - 213 WP_000087756.1 cold shock-like protein CspF -
FORC28_RS15120 2866233..2866448 + 216 WP_000066483.1 cold shock-like protein CspB -
FORC28_RS33930 2866812..2866982 - 171 WP_001405948.1 putative zinc-binding protein YnfU -
FORC28_RS15125 2867202..2867417 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - galU / paa / espK / espW / espM2 2732068..2906285 174217


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T57024 WP_000323025.1 NZ_CP012693:2862154-2862441 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T57024 NZ_CP012693:2862154-2862441 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT57024 WP_000534858.1 NZ_CP012693:2861915-2862154 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT57024 NZ_CP012693:2861915-2862154 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References