Detailed information of TA system TA057020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2301157..2301628
• Replicon: chromosome
• Accession: NZ_CP012693
• Organism: Escherichia coli strain FORC_028

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: FORC28_RS11620
• Protein ID: WP_001303511.1
• Coordinates: 2301157..2301435 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: FORC28_RS11625
• Protein ID: WP_001302048.1
• Coordinates: 2301437..2301628 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC28_RS11595	2296807..2299458	-	2652	WP_000105101.1	exodeoxyribonuclease VIII	-
FORC28_RS11600	2299557..2299832	-	276	WP_001307773.1	hypothetical protein	-
FORC28_RS11605	2299906..2300082	-	177	WP_000245534.1	hypothetical protein	-
FORC28_RS11610	2300076..2300297	-	222	WP_000560218.1	killing protein KilR	-
FORC28_RS11615	2300718..2300870	-	153	WP_001427316.1	DUF1391 family protein	-
FORC28_RS11620	2301157..2301435	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC28_RS11625	2301437..2301628	-	192	WP_001302048.1	hypothetical protein	Antitoxin
FORC28_RS32160	2301649..2302020	-	372	WP_001169687.1	hypothetical protein	-
FORC28_RS11635	2302118..2302420	+	303	WP_000172738.1	transcriptional regulator	-
FORC28_RS11640	2302417..2302842	+	426	WP_000693943.1	Rha family transcriptional regulator	-
FORC28_RS11645	2302865..2303827	+	963	WP_077886425.1	helix-turn-helix domain-containing protein	-
FORC28_RS11650	2303834..2304575	+	742	Protein_2235	ATP-binding protein	-
FORC28_RS11655	2304601..2305362	+	762	WP_000450872.1	DUF1627 domain-containing protein	-
FORC28_RS11660	2305395..2305676	+	282	WP_000603384.1	DNA-binding protein	-
FORC28_RS11665	2305673..2305900	+	228	WP_000699809.1	hypothetical protein	-
FORC28_RS11670	2305893..2306204	+	312	WP_001289673.1	hypothetical protein	-
FORC28_RS11675	2306332..2306550	+	219	WP_000683609.1	DUF4014 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2280801..2339490	58689

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T57020 WP_001303511.1 NZ_CP012693:c2301435-2301157 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T57020 NZ_CP012693:c2301435-2301157 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT57020 WP_001302048.1 NZ_CP012693:c2301628-2301437 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT57020 NZ_CP012693:c2301628-2301437 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0