Detailed information of TA system TA057005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1437641..1438266
• Replicon: chromosome
• Accession: NZ_CP012693
• Organism: Escherichia coli strain FORC_028

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FORC28_RS07200
• Protein ID: WP_000911330.1
• Coordinates: 1437868..1438266 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FORC28_RS07195
• Protein ID: WP_000450524.1
• Coordinates: 1437641..1437868 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC28_RS07165	1433444..1433914	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
FORC28_RS07170	1433914..1434486	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FORC28_RS07175	1434632..1435510	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FORC28_RS07180	1435527..1436561	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FORC28_RS07190	1436774..1437487	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FORC28_RS07195	1437641..1437868	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FORC28_RS07200	1437868..1438266	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FORC28_RS07205	1438413..1439276	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
FORC28_RS07210	1439291..1441306	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
FORC28_RS07215	1441380..1442078	+	699	WP_000679812.1	esterase	-
FORC28_RS07220	1442188..1442388	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T57005 WP_000911330.1 NZ_CP012693:1437868-1438266 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T57005 NZ_CP012693:1437868-1438266 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT57005 WP_000450524.1 NZ_CP012693:1437641-1437868 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT57005 NZ_CP012693:1437641-1437868 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33