Detailed information of TA system TA056993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2223213..2223742
• Replicon: chromosome
• Accession: NZ_CP012692
• Organism: Staphylococcus aureus strain FORC_027

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FORC27_RS11380
• Protein ID: WP_000621175.1
• Coordinates: 2223213..2223575 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FORC27_RS11385
• Protein ID: WP_000948331.1
• Coordinates: 2223572..2223742 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC27_RS11355	2220192..2220962	-	771	WP_001041106.1	RNA polymerase sigma factor SigB	-
FORC27_RS11360	2220937..2221416	-	480	WP_044421339.1	anti-sigma B factor RsbW	-
FORC27_RS11365	2221418..2221744	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FORC27_RS11370	2221863..2222864	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FORC27_RS11380	2223213..2223575	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FORC27_RS11385	2223572..2223742	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FORC27_RS11390	2223827..2224975	-	1149	WP_001281154.1	alanine racemase	-
FORC27_RS11395	2225041..2225400	-	360	WP_000581197.1	holo-ACP synthase	-
FORC27_RS11400	2225404..2225895	-	492	WP_001205912.1	PH domain-containing protein	-
FORC27_RS11405	2225882..2227465	-	1584	WP_001294626.1	PH domain-containing protein	-
FORC27_RS11410	2227458..2227937	-	480	WP_001287087.1	hypothetical protein	-
FORC27_RS11415	2228145..2228705	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T56993 WP_000621175.1 NZ_CP012692:c2223575-2223213 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T56993 NZ_CP012692:c2223575-2223213 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT56993 WP_000948331.1 NZ_CP012692:c2223742-2223572 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT56993 NZ_CP012692:c2223742-2223572 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1