Detailed information of TA system TA056981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1683204..1684821
• Replicon: plasmid unnamed
• Accession: NZ_CP012688
• Organism: Ralstonia solanacearum strain UY031

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A850FK36
• Locus tag: RSUY_RS21895
• Protein ID: WP_003265839.1
• Coordinates: 1683475..1684821 (+)
• Length: 449 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: RSUY_RS21890
• Protein ID: WP_003265838.1
• Coordinates: 1683204..1683470 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSUY_RS21860	1678431..1678907	+	477	WP_003265832.1	RidA family protein	-
RSUY_RS21865	1679008..1679844	+	837	WP_003265833.1	AraC family transcriptional regulator	-
RSUY_RS21870	1679847..1680965	+	1119	WP_020957649.1	dienelactone hydrolase	-
RSUY_RS21875	1681004..1681387	-	384	WP_003265835.1	VOC family protein	-
RSUY_RS21880	1681644..1682078	+	435	WP_003277354.1	PGDYG domain-containing protein	-
RSUY_RS21885	1682094..1682945	-	852	WP_003265837.1	hypothetical protein	-
RSUY_RS21890	1683204..1683470	+	267	WP_003265838.1	helix-turn-helix domain-containing protein	Antitoxin
RSUY_RS21895	1683475..1684821	+	1347	WP_003265839.1	type II toxin-antitoxin system HipA family toxin	Toxin
RSUY_RS21900	1684916..1685812	+	897	WP_003265840.1	DUF2236 domain-containing protein	-
RSUY_RS21905	1685816..1687387	-	1572	WP_003265841.1	MFS transporter	-
RSUY_RS21910	1687480..1688430	-	951	WP_003265842.1	LysR family transcriptional regulator	-
RSUY_RS21915	1688574..1689200	+	627	WP_003265843.1	FMN-dependent NADH-azoreductase	-
RSUY_RS25000	1689348..1689494	-	147	WP_003277363.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgG / fliM / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1999545	1999545

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (11-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-67)

Sequences

Toxin

Protein

• Length: 449 a.a.
• Molecular weight: 49589.91 Da
• Isoelectric Point: 7.8551

>T56981 WP_003265839.1 NZ_CP012688:1683475-1684821 [Ralstonia solanacearum]
MGRRSHTRALGLWMNGTHVGRWELHPHQGDVLKYAASWVAAPEGRPLSLSLPFTPDNAPHKGPAVRAYFENLLPDSRDIR
ERIARRFQAGTTEAFDLLEQIGRDCIGALQVLPADMAPPATGDIDAEPLTEAQIAQTLRETVAPPPLGGMPEDDGHFRIS
IAGAQEKTAFLQIDGHWYRPRGATPTSHIFKLPMGLVGNMKLDLSESVENEWLCALVLRAYGLPVAHCRPLVFEDQKVLA
VERFDRMWWDRGGKRLIRLPQEDMCQATATPPHLKYEADGGPGIDRIMGLLDGSAHREADRLTLFRSQVLFWMLCAPDGH
AKNFSLALRPGGAYALTPLYDVMSAYPLLGEGPGKLSPHRIRLAMAVRSKNAHWKMQDIQRRHWLGLGQRYGIVTSDGAD
AASIVDNLVARTPEVTRTVRAMLPEGFPQPLAERILQGLRHAADKLAG

Nucleotide

• Length: 1347 bp

>T56981 NZ_CP012688:1683475-1684821 [Ralstonia solanacearum]
ATGGGGCGCCGGTCGCATACGCGGGCCCTCGGGCTCTGGATGAATGGCACCCATGTCGGCCGCTGGGAGCTGCACCCGCA
CCAGGGCGACGTGCTGAAGTACGCGGCATCCTGGGTGGCCGCGCCCGAGGGGCGGCCCCTGTCGCTGTCCCTGCCGTTCA
CGCCAGACAACGCGCCCCACAAGGGCCCGGCGGTCCGGGCGTATTTCGAGAACCTCCTGCCGGACAGCCGCGACATCCGC
GAGCGCATCGCGCGCCGTTTCCAGGCCGGGACAACCGAGGCGTTCGACCTGCTGGAGCAGATCGGCCGCGACTGCATCGG
CGCCCTGCAAGTGCTGCCGGCCGACATGGCCCCACCGGCCACGGGCGACATCGACGCGGAACCGCTAACCGAGGCGCAGA
TCGCGCAGACGCTGCGGGAGACCGTCGCCCCGCCACCCTTGGGCGGCATGCCGGAAGACGACGGCCATTTCCGCATCTCG
ATCGCCGGCGCGCAGGAAAAGACGGCCTTCCTCCAGATCGACGGACACTGGTACCGCCCCCGGGGCGCGACGCCGACCAG
CCACATCTTCAAGCTGCCGATGGGGCTGGTCGGCAACATGAAGCTGGACCTGAGCGAGTCGGTGGAAAACGAATGGCTGT
GCGCCCTGGTGCTGCGGGCGTATGGCCTGCCCGTCGCGCACTGCCGGCCGCTGGTGTTCGAAGACCAGAAGGTGCTCGCC
GTGGAGCGGTTCGACCGCATGTGGTGGGACCGCGGCGGCAAACGGCTCATCCGCCTGCCGCAGGAAGACATGTGCCAGGC
CACGGCAACGCCCCCGCACCTGAAGTACGAGGCCGACGGCGGCCCCGGCATCGACCGCATCATGGGTTTGCTCGACGGCT
CCGCGCACCGGGAGGCCGACCGCCTGACGCTGTTCCGCAGCCAGGTGCTGTTCTGGATGCTGTGTGCGCCCGATGGCCAT
GCGAAGAACTTCAGCCTCGCGCTGCGTCCCGGCGGCGCCTACGCGCTCACGCCGCTGTACGACGTGATGTCGGCCTACCC
GCTGCTGGGCGAGGGGCCGGGCAAGCTGTCGCCGCACCGGATCCGGCTGGCGATGGCCGTCCGCTCGAAGAATGCGCACT
GGAAAATGCAGGACATCCAGCGTCGCCACTGGCTGGGCCTGGGCCAACGCTACGGCATCGTCACGTCCGATGGCGCGGAT
GCCGCCTCCATCGTGGACAACCTGGTGGCCCGGACGCCCGAGGTGACCCGTACCGTGCGCGCGATGCTGCCGGAAGGCTT
CCCGCAGCCGCTGGCCGAGCGCATTCTGCAGGGGCTGCGGCACGCCGCGGACAAACTCGCGGGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9668.20 Da
• Isoelectric Point: 10.9487

>AT56981 WP_003265838.1 NZ_CP012688:1683204-1683470 [Ralstonia solanacearum]
MNEFIVRTPEQLPAILKGFRKQAGLSQAELAARMGMRQQTLSALERNAENVSAGRLMRLLSVLGVELVLRKPDAAGEPGR
PASDQPQW

Nucleotide

• Length: 267 bp

>AT56981 NZ_CP012688:1683204-1683470 [Ralstonia solanacearum]
ATGAACGAGTTCATCGTACGCACCCCCGAGCAGTTGCCGGCGATCCTCAAGGGTTTCCGCAAACAGGCCGGGCTGAGCCA
GGCCGAACTGGCCGCCCGGATGGGCATGCGCCAGCAAACCCTCTCCGCGTTGGAGCGCAACGCAGAAAACGTGAGCGCCG
GGCGGCTGATGCGCCTGCTGTCGGTGCTGGGCGTGGAGCTGGTGCTGCGCAAACCCGACGCCGCCGGCGAGCCCGGCCGC
CCGGCGTCCGACCAGCCGCAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A850FK36