Detailed information of TA system TA056933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1628021..1628820
• Replicon: chromosome
• Accession: NZ_CP012674
• Organism: Proteus mirabilis strain CYPM1

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: AOC00_RS07450
• Protein ID: WP_004247966.1
• Coordinates: 1628296..1628820 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: AOC00_RS07445
• Protein ID: WP_004247964.1
• Coordinates: 1628021..1628299 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOC00_RS07420 (AOC00_07425)	1623371..1624453	+	1083	WP_004242680.1	peptide chain release factor 1	-
AOC00_RS07425 (AOC00_07430)	1624453..1625301	+	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
AOC00_RS07430 (AOC00_07435)	1625279..1626094	+	816	WP_004242688.1	invasion regulator SirB1	-
AOC00_RS07435 (AOC00_07440)	1626152..1627006	+	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
AOC00_RS07440 (AOC00_07445)	1627014..1627943	+	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
AOC00_RS07445 (AOC00_07450)	1628021..1628299	+	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
AOC00_RS07450 (AOC00_07455)	1628296..1628820	+	525	WP_004247966.1	hypothetical protein	Toxin
AOC00_RS07455 (AOC00_07460)	1628901..1630601	-	1701	WP_017628159.1	C4-dicarboxylic acid transporter DauA	-
AOC00_RS07465 (AOC00_07470)	1631565..1632506	+	942	WP_004242695.1	glyoxylate/hydroxypyruvate reductase GhrA	-
AOC00_RS18120	1632697..1633422	+	726	WP_237073880.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T56933 WP_004247966.1 NZ_CP012674:1628296-1628820 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T56933 NZ_CP012674:1628296-1628820 [Proteus mirabilis]
ATGACAATACCAAATTGGCACGAAGAAGCGATCAGTAAAAAACATAATAGAAATGGATTTAATTGTGGCGATGCAGTACT
AAACCAATTTCTGTATCGCCATGCAAGACAAAATCATGAGAATGGTAGTGCGAAAACATATTTAGCTATCAATAATAGTA
ATAATATTATATTAGGTTATTACAGCTTATGTCCTGCTTCTATTGAGTTCGAAAGAACGCCCGAATTGATCAAACATGGC
TTAGCGCGTCATGATATCCCTGTTTTTAAACTTGCACGACTAGCTACGGATCTATCCGTTCAAGGAAAAGGTTTGGGTGG
GCAATTATTGCTTGCAGCAGGAAGGCGTTGTCTTTCCGTTGCTAGTGAATTGGGAGGCGTTGCTTTACTTATAGATGCTA
AAAATGAACGAGTCGCTAATTGGTATATTAGCTACGGGGCAATACCATTATTAGATACCCCTCTTTCTTTATTAATTTCC
TTTAAAACCATTTATACTGCACTTTCAATGGCAAATAAAATATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT56933 WP_004247964.1 NZ_CP012674:1628021-1628299 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT56933 NZ_CP012674:1628021-1628299 [Proteus mirabilis]
ATGGCTCAAGCACTAGTAGAAAACAGTGAACGAGTGTCAATTCGTATCACTACAGAAGATAAATCACTGCTGGTTCGTGC
TGCTAGTTTGGAAAATACAAACTTAACAGAGTTTGTACTTCGTAATATTGTACCTGTAGCTCGGCAAGTTATTGAAAATA
ATGAGCGTTTAAAATTATCAGAAAAAGACACATTACGTGTTATGGAACTTTTAGATAATCCACCAAAGCCTAATGCTAAA
CTACTTGCAGCGGCTTTTGCTCTACCGGACAATGCATGA

Structures

Antitoxin

• AlphaFold DB: B4EVS2