Detailed information of TA system TA056900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 62887..63263
• Replicon: plasmid pSF-088-1
• Accession: NZ_CP012636
• Organism: Escherichia coli strain SF-088

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: AN203_RS25720
• Protein ID: WP_001514250.1
• Coordinates: 62887..63069 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: AN203_RS25725
• Protein ID: WP_001324224.1
• Coordinates: 63066..63263 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN203_RS25710	60886..61920	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
AN203_RS25715	62480..62788	-	309	WP_000377483.1	hypothetical protein	-
AN203_RS25720	62887..63069	-	183	WP_001514250.1	hypothetical protein	Toxin
AN203_RS25725	63066..63263	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
AN203_RS25730	63945..65219	+	1275	WP_000489608.1	HlyD family secretion protein	-
AN203_RS25735	65194..67308	+	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
AN203_RS25740	67478..67789	-	312	WP_001259758.1	colicin V	-
AN203_RS29995	67767..68003	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / dfrA5 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..149683	149683

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6961.10 Da
• Isoelectric Point: 4.5349

>T56900 WP_001514250.1 NZ_CP012636:c63069-62887 [Escherichia coli]
MMEIFWTMLTSQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T56900 NZ_CP012636:c63069-62887 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGACCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT56900 WP_001324224.1 NZ_CP012636:c63263-63066 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT56900 NZ_CP012636:c63263-63066 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA