Detailed information of TA system TA056897

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4482774..4483608
• Replicon: chromosome
• Accession: NZ_CP012635
• Organism: Escherichia coli strain SF-088

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: AN203_RS22645
• Protein ID: WP_000854820.1
• Coordinates: 4483231..4483608 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: AN203_RS22640
• Protein ID: WP_001285610.1
• Coordinates: 4482774..4483142 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN203_RS22605	4478495..4479175	+	681	WP_001278649.1	WYL domain-containing protein	-
AN203_RS22610	4479326..4480003	+	678	WP_001097565.1	hypothetical protein	-
AN203_RS22615	4480009..4480242	+	234	WP_000883181.1	DUF905 family protein	-
AN203_RS22620	4480332..4481150	+	819	WP_001175155.1	DUF945 domain-containing protein	-
AN203_RS30115	4481176..4481316	-	141	WP_000997937.1	hypothetical protein	-
AN203_RS22625	4481416..4481895	+	480	WP_000706978.1	antirestriction protein	-
AN203_RS22630	4481910..4482386	+	477	WP_001186193.1	RadC family protein	-
AN203_RS22635	4482473..4482694	+	222	WP_000692347.1	DUF987 domain-containing protein	-
AN203_RS22640	4482774..4483142	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AN203_RS22645	4483231..4483608	+	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
AN203_RS29575	4483605..4483802	+	198	Protein_4306	hypothetical protein	-
AN203_RS29580	4483830..4484027	+	198	WP_085949158.1	DUF957 domain-containing protein	-
AN203_RS22660	4484112..4484672	+	561	Protein_4308	DUF4942 domain-containing protein	-
AN203_RS22670	4485507..4487045	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T56897 WP_000854820.1 NZ_CP012635:4483231-4483608 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T56897 NZ_CP012635:4483231-4483608 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT56897 WP_001285610.1 NZ_CP012635:4482774-4483142 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT56897 NZ_CP012635:4482774-4483142 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6