Detailed information of TA system TA056871

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4380092..4380926
• Replicon: chromosome
• Accession: NZ_CP012633
• Organism: Escherichia coli strain SF-166

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: AN204_RS21650
• Protein ID: WP_000854820.1
• Coordinates: 4380549..4380926 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: AN204_RS21645
• Protein ID: WP_001285610.1
• Coordinates: 4380092..4380460 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN204_RS21610	4375813..4376493	+	681	WP_001278649.1	WYL domain-containing protein	-
AN204_RS21615	4376644..4377321	+	678	WP_001097565.1	hypothetical protein	-
AN204_RS21620	4377327..4377560	+	234	WP_000883181.1	DUF905 family protein	-
AN204_RS21625	4377650..4378468	+	819	WP_001175155.1	DUF945 domain-containing protein	-
AN204_RS21630	4378734..4379213	+	480	WP_000706978.1	antirestriction protein	-
AN204_RS21635	4379228..4379704	+	477	WP_001186193.1	RadC family protein	-
AN204_RS21640	4379791..4380012	+	222	WP_000692347.1	DUF987 domain-containing protein	-
AN204_RS21645	4380092..4380460	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AN204_RS21650	4380549..4380926	+	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
AN204_RS27935	4380923..4381120	+	198	Protein_4123	hypothetical protein	-
AN204_RS27940	4381148..4381345	+	198	WP_085949158.1	DUF957 domain-containing protein	-
AN204_RS21665	4381430..4381990	+	561	Protein_4125	DUF4942 domain-containing protein	-
AN204_RS21680	4382824..4384362	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T56871 WP_000854820.1 NZ_CP012633:4380549-4380926 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T56871 NZ_CP012633:4380549-4380926 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT56871 WP_001285610.1 NZ_CP012633:4380092-4380460 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT56871 NZ_CP012633:4380092-4380460 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6