Detailed information of TA system TA056868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4052029..4052605
• Replicon: chromosome
• Accession: NZ_CP012633
• Organism: Escherichia coli strain SF-166

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: AN204_RS19905
• Protein ID: WP_001297643.1
• Coordinates: 4052318..4052605 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: AN204_RS19900
• Protein ID: WP_000063156.1
• Coordinates: 4052029..4052331 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN204_RS19885	4048625..4050775	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
AN204_RS19890	4050825..4051028	+	204	WP_000467859.1	YbdD/YjiX family protein	-
AN204_RS19895	4051039..4051995	+	957	WP_001297640.1	GTPase	-
AN204_RS19900	4052029..4052331	-	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
AN204_RS19905	4052318..4052605	-	288	WP_001297643.1	BrnT family toxin	Toxin
AN204_RS19910	4052804..4053501	-	698	Protein_3798	IS1 family transposase	-
AN204_RS26280	4053558..4053653	+	96	Protein_3799	dienelactone hydrolase family protein	-
AN204_RS19920	4053799..4055031	+	1233	WP_001339512.1	multidrug efflux MFS transporter MdtM	-
AN204_RS19925	4055072..4055599	+	528	Protein_3801	DUF445 family protein	-
AN204_RS27320	4055647..4057184	+	1538	WP_085953974.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4052804..4053013	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T56868 WP_001297643.1 NZ_CP012633:c4052605-4052318 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T56868 NZ_CP012633:c4052605-4052318 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT56868 WP_000063156.1 NZ_CP012633:c4052331-4052029 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT56868 NZ_CP012633:c4052331-4052029 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9

Antitoxin

• AlphaFold DB: A0A829G8Z0